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Showing 3 results for Vahdati

Lida Momeni, Akbar Vahdati, Taji Afrooz,
Volume 7, Issue 1 (Spring and Summer 2003)
Abstract

Human recombinant erythropoietin is a synthetic hormone for treatment of some kinds of anemia. In this study, the effect of this hormone was investigated on blood cell changes in adult and immature rats. For this purpose, the hormone was administered at doses of 50, 100, and 200 IU/Kg (s.c.) twice a week for a period of 6 weeks. Then, the number of red blood cells (RBC), subtypes of white blood cells (WBC), and platelets were counted using H1 counter. For statistical analysis, ANOVA (followed by Tukey post-hoc test) and paired student's t-test were used. The results showed that the number of RBC increases by 15 % as the dose of the hormone increases in both groups of rats. In addition, number of WBC decreased by 10 % at doses of 50 and l00 IU/Kg of the hormone. There was also a decrease in the number of neutrophils (25 %), lymphocytes (16.65 %), and basophils (30 %), and an increase in the number of eosinophil (50 %). Furthermore, number of monocytes and platelets decreased (10%) as the dose of the hormone increased. These results were alike for both groups of the rats irrespective of their age. The effect of rHuEpo is probably mediated through its stimulatory receptors on the surface of hematopoietic stem cells. Meanwhile, it is also possible that this hormone could inhibit the synthesis of neutrophil growth factors, interleukins with stimulatory effect on RBC maturation, and platelet-stimulatory factors.
Shohreh Iranmanesh, Akbar Vahdati, Taji Afrouz,
Volume 10, Issue 2 (Summer 2006)
Abstract

Introduction: Estrogen is one of the female sex hormones that in addition to its reproductive role, has favorable fects on the metabolism of blood lipids and lipoproteins and by this way decreases the coronary heart disease. n the other hand, phytoestrogens are phytochemical substances that have effects like estrogens. Isoflavones, hich the most important of them is genistein, are the most common form of phytoestrogens. In this research, the fects of one phytoestrogenic compounds named genistein and one estrogenic compound named ethinyl estradiol ere compaired. The purpose of this comparison was to study of replacement possibility of phytoestrogens in RT period for elimination of side effects of estrogenic compound. Methods: First the rats were fed for 2 months with high cholestrol diet (2% cholestrol, 0/5% cholic acid). fter the first blood test ensured the hypercholestrolemic condition, the rats were treated for 2 months with ethinyl stradiol and genistein besides of high cholestrol diet. After the end of the second period and serum preparation om the blood samples disignated total cholestrol, triglyceride, HDL, LDL and VDL were measured and the sults were statistically analyzed. Results: According to the obtained results of the first period of experiment, the usage of high cholestrol diet aused meaningful increase in total cholestrol and LDL as it made rats hypercholestrolemic. According to the sults of second period of experiment, the usage of ethinyl estradiol reduced the level of serum total cholestrol nd LDL of hypercholestrolemic rats, that was because of the effect of estrogen on increase of LDL catabolism. hereas, it did not produce meaningful changes in triglyceride, HDL and VDL. Usage of genistein in isolated rm had no benefical effect on blood lipids and lipoproteins, Because hypocholestrolemic property of isoflavone epends on matrix that is beside it.
Parva Nasimi, Mohammad Reza Tabandeh, Akbar Vahdati, Saeed Khatamsaz,
Volume 19, Issue 3 (September 2015)
Abstract

Introduction: Busulfan as a chemotherapeutic agent causes testicular germinal epithelium depletion and cytotoxicity in germ cells. The aim of this study was to assess antioxidant status, reactive oxygen species (ROS) generation and apoptosis-related genetic markers of adult male mouse sperm following busulfan treatment. Materials and Methods: Forty adult NMRI mice (30 ± 5 g) were divided into two groups. Control and busulfan treated group were administered with 100 &muL dimethyl sulfoxide and 3.2 mg/kg/day busulfan for 4 days, respectively. The superoxide dismutase and glutathione peroxidase assays were used for analyzing antioxidant status. Then, the levels of Bcl-2 family gene expression, lipid peroxidation and cytotoxicity were evaluated by Real-Time PCR, thiobarbituric and lactate dehydrogenase assays, respectively. Results: The results showed significant decrease on antioxidant status, increase on lipid peroxidation and lactate dehydrogenase in epididymal sperm and testis of busulfan treated mice in comparison with control (P< 0.05). Real Time PCR demonstrated significantly increased-Bax gene expression and decreased-Bcl-2 gene expression in epididymal sperm of treated group (P< 0.05). Conclusion: The high levels of lipid peroxidation and lactate dehydrogenase revealed increased-ROS and severe cytotoxicity in epididymal sperm and testis tissue following busulfan treatment at clinical dose. The oxidative stress and increased-ROS may induce Bcl-2 family gene expression-related apoptosis following busulfan therapy in normal cells.



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