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Showing 5 results for Radahmadi

Nastaran Eidelkhani, Maryam Radahmadi, Laleh Rafiee, Mahsa Gharzi, Hojjatallah Alaei, Parham Reisi,
Volume 19, Issue 3 (September 2015)

Introduction: Although the initial hypothesis for the action of doxepin was based on the inhibition of the reuptake of neurotransmitters, it has been suggested that it may also involve other mechanisms. Therefore, this study aims to investigate the effect of doxepin on spatial memory, tumor necrosis factor alpha (TNF-&alpha) level, expression of pro-apoptotic (Bad and Bax) and anti-apoptotic (Bcl-2) genes in the rat hippocampus. Materials and Methods: Male rats were divided randomly into three groups the control, the doxepin 1 and 5 mg/kg, respectively). Rats received i.p injection of doxepin for 21 days. Spatial memory was evaluated by Morris water maze test. Then the hippocampi were dissected for measurement of the expression of Bcl2, Bad and Bax genes and the TNF-&alpha level. Results: Our results showed no significant effects of doxepin on spatial memory. Doxepin significantly decreased expression of Bad gene, but had no significant/considerable effects on Bcl2 and Bax gene expression. Also, the ratio of TNF-&alpha to total protein (%) did not show significant differences in the rat hippocampus. Conclusion: These results did not show any significant impact of doxepin on the factors affecting the neuronal functions in intact animals. However, Since a significant reduction in the hippocampal Bad mRNA levels was observed It is our assumption that doxepin has neuroprotective effects.

Ali Ahmad Azadbakht, Nastaran Eidelkhani, Mohammad Kazemi, Maryam Radahmadi, Parham Reisi,
Volume 20, Issue 4 (December 2016)

Introduction: Stress is associated with neurological and cognitive disorders. It has been suggested that doxepin, in addition to its influence on the content of neurotransmitters, has probable neuroprotective effects as well. Therefore, the aim of this study was to investigate the effects of doxepin on synaptic plasticity and brain-derived neurotrophic factor (BDNF) gene expression in the rat hippocampus following repeated restraint stress. Methods: Male Wistar rats were divided into the control, the stress and the stress-doxepin 1 and 5 mg/kg groups. Stress was induced 6 hours/day for 21 days. Rats received daily ip injection of doxepin before induction of stress. Long-term potentiation (LTP) was induced in hippocampal dentate gyrus following stimulation of perforant pathway and then field excitatory postsynaptic potential was evaluated. Hippocampal gene expression of BDNF was measured by Real-Time PCR. Results: Stress impaired LTP induction, but both doses of doxepin prevented those damages. Stress significantly decreased the expression of BDNF gene, but doxepin in both doses, increased it significantly. Conclusion: The present results suggested that doxepin can prevented the harmful effects of stress on synaptic plasticity which may be related to changes in BDNF gene expression.

Maryam Radahmadi, Azadehalsadat Hosseini Dastgerdi, Neda Fallah, Hojjatallah Alaei,
Volume 21, Issue 3 (September 2017)

Introduction: Stress is a main factor influencing brain functions as revealed by the electroencephalogram (EEG) recordings. Moreover, different stress durations seemingly cause perturbations in brain waves and lead to mental disorders. This study investigates the effects of acute, sub-chronic and chronic stress on EEG in rats.  Methods: Twenty-eight Wistar adult male rats were randomly allocated to one control and three experimental groups subjected to 6 hr/day of acute (1d), sub-chronic (7d) and chronic (21d) stress. At the end of each period, 20 minutes of EEG recording was taken of each subject. Results: Percentages of delta, theta and alpha frequencies of the baseline in the chronic stress group showed significant differences from those of the control (P<0.05). Theta waves increased in the chronic stress group compared to the acute and sub-chronic (P<0.05 and P<0.01; respectively) ones. This is while, compared to the control, the acute and sub-chronic stress groups exhibited significantly increased percentages of beta waves (P<0.05 in both). Conclusion: The data indicate that different stress durations have different impacts on the EEG rhythm. Acute and sub-chronic stress durations led to changed cortical activity, indicating the inability of the subjects to cope with the stress imposed. Also, chronic stress caused irregularities in the EEG rhythm (delta, theta and alpha waves). EEG recording seems to be useful for measuring stress levels and for predicting abnormalities due to different stress durations.

Mina Sadat Izadi, Maryam Radahmadi, Maedeh Ghasemi, Atefeh Rayatpour,
Volume 22, Issue 2 (June 2018)

Introduction: Psychological stresses influence brain functions such as learning and memory. Environmental factors like types and durations of stress affect brain responsiveness. This study investigated the effects of two subchronic social and isolation stresses on learning, memory, adrenal glands weight and corticosterone levels in the hippocampus and frontal cortex. Methods: Eighteen male rats were randomly allocated into three experimental groups: control, social stress and isolation stress groups. Rats were under stresses for 7 days. Latency of entrance into the dark room was evaluated as brain function, using the passive avoidance test before inducing of electrical shock (as initial latency) and on days 1, 3, 5 and 7 after foot shock. In addition, corticosterone levels were measured in the homogenized hippocampus and frontal cortex. Results: The latencies of days 1, 3 and 5 were significantly lower in an isolation stress group than the control group. The latency of day 7 significantly decreased in social and isolation stress groups, compared to the control group. The adrenal glands weight showed significant enhancements in social and isolation stress groups, compared to the control group. Although, the weight of the adrenal glands significantly increased in an isolation stress group, compared to the social stress group. There was a significant enhancement in the corticosterone levels in the hippocampus, but not frontal cortex in isolation stress group. Conclusion: It was concluded that subchronic isolation stress severely deteriorated brain functions (learning and memory) compared to the subchronic social stress. In addition, isolation stress affected corticosterone levels in the hippocampus more than frontal cortex.

Fatemeh Khani, Maryam Radahmadi, Hojjatallah Alaei, Elahe Jafari,
Volume 22, Issue 4 (December 2018)

Introduction: Certain types of chronic mental stress impair memory. On the other hand, crocin is introduced in the medical literature as an effective component of saffron with remedial effects on memory impairment. This study investigated the effects of crocin on spatial and cognitive memories, locomotor activity, novel recognition conditions and serum corticosterone levels in rats under chronic isolation stress. Methods: Male rats were randomly allocated to the five groups of control, sham, isolation stress (St.I), St.I-C30 and St.I-C60. The latter two groups were exposed to chronic isolation stress (6h/day) receiving two levels of crocin (30 and 60 mg/kg, respectively) over a period of 21 days. The object location and novel object recognition tests (OLT and NOR) were used to evaluate spatial and cognitive memories, respectively. Results: The OLT results revealed that chronic isolation stress led to significantly decreased locomotor activity in all the stressed groups; the NOR test, however, yielded similar results only in the St.I group. Moreover, isolation stress was found to lead significant declines in spatial and cognitive memories. Finally, crocin administration led to improvements in impaired memory in St.I-C30 and St.I-C60 groups. There were significant enhancements in serum corticosterone levels in the St.I and St.I-C30 groups as compared with the control group. Conclusion: Our findings indicate that spatial and cognitive memory impairments are strongly affected by isolation stress and crocin especially at its high dose of 60 mg/kg, exhibits better protective effects against cognitive memory deficit induced by chronic isolation stress.

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