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Showing 4 results for Nasimi

Ali Nasimi, Masoumeh Hatam,
Volume 10, Issue 3 (Fall 2006)
Abstract

Introduction: We previously shown that microinjection of glutamate into the hDB of rat elicited cardiovascular depressive responses. Microinjection of AP5 (an NMDA receptor antagonist, 2.5 mM, 50 nl) and CNQX (an AMPA receptor antagonist, 1 mM, 50 nl) caused no significant changes in the blood pressure and heart rate. Microinjection of bicuculline (BMI: a GABAA receptor antagonist, 1 mM, 50 nl) resulted in the increased blood pressure and heart rate. In this study we investigated the possible interaction of GABAergic and glutaminergic systems by coinjection of the antagonists of both systems. Methods: Experiments were performed on 27 urethane anesthetized rats. Drugs were microinjected into the hDB using micropipettes. The arterial pressure and heart rate were continuously recorded and repeated measures ANOVA was used for data analysis. Results: Our results showed that coinjection of 50 nl of BMI (1mM ) and AP5 (2.5 mM) significantly (P < 0. 01) decreased the presser effects of BMI. Also, coinjection of 50 nl of BMI (1 mM) and CNQX (1 mM) significantly (P < 0.01) decreased the presser effects of BMI. Microinjection of the doses of BMI and two glutamate receptor antagonists produced the same results. Conclusion: These findings suggest that the cardiovascular effects of the blockade of GABAergic system depend on the activation of local NMDA and AMPA receptors. A possible explanation for the results is that, GABAergic system tonically inhibits the glutaminergic neurons.
Maesoumeh Hatam, Ali Nasimi,
Volume 10, Issue 4 (Winter 2007)
Abstract

Introduction: The Bed nucleus stria terminalis (BST) is a part of the limbic system. It was recently shown that chemical stimulation of the BST by L-glutamate elicited cardiovascular depressive responses. In the present study, we have investigated the possible cardiovascular role of the GABAergic receptors in BST by microinjection of its agonist and antagonists. Methods: Experiments were performed on 21 anaesthetized rats. Drugs were microinjected into the BST in volume of 50 nl using streotaxic apparatus. Blood pressure and heart rate were recorded before and throughout each experiment. The averages of maximum changes in the arterial pressure and heart rate were compared with control group and with its average in before injections using student t-test and paired t-test, respectively. Results: GABAA receptor antagonist, bicuculline met iodide (BMI, 1 mM), increased both arterial pressure (+14.2±3.08) and heart rate (+9.8± 2.5) (p<0.05). Muscimole, a GABAA agonist (5 mM), caused a significant decrease of the arterial pressure (-10.2 ±4.1) and heart rate (-20.3±9.40) (p<0.01). However, microinjection of phaclofen (5 mM), a GABAB receptor antagonist caused small unsignificant changes of the heart rate and blood pressure. Conclusion: GABAergic inhibitory neurons of the BST seems to cause decrease in the blood pressure and heart rate by GABAA but not GABAB receptors.
Ali Nasimi, Ali Mohammad Moradi, Mardomak Ravari, Fatemeh Kharazmi, Masoumeh Hatam,
Volume 12, Issue 4 (Winter 2009)
Abstract

Introduction: The bed nucleus of the stria terminalis (BST) is a limbic structure which is involved in cardiovascular regulation and baroreflex modulation. The presence of cholinergic synaptic terminalis with high level of muscarinic receptors in the BST has been demonstrated. This study was performed to find the role of the cholinergic muscarinic receptor in cardiovascular response and baroreflex activity in urethane anesthetized rat. Methods: Acetylcholine (Ach, 3, 6 nmol in 50 nl) was microinjected unilaterally into the BST of 53 urethane anesthetized male rats. Femoral artery and vein were cannulated to record the blood pressure (AP) and heart rate (HR), respectively. The maximum average changes in the mean arterial pressure (MAP) and (HR), were compared with control group and before injection using t-test and paired t-test, respectively. To evaluate baroreflex activity, bradycardia values corresponding to progressive 20 mmHg increases in MAP were determined. The slope of the linear regression curves was calculated and compared to before injection using ANOVA repeated measure. Results: Microinjection of Ach into the dorsal, lateral and ventral portion of the BST resulted in an increase of AP (20.69 ± 1.8 mmHg, p<0.01) with no significant changes of the HR. The pressor response evoked by Ach was blocked by microinjection of atropine into the BST. However, atropine did not affect the bradycardia reflex evoked by increased blood pressure caused by intravenous phenylephrine injection. Microinjection of cobalt chloride into the BST did not affect the baseline AP and HR but significantly increased bradycardic response to lower pressure changes (less than 40 mm Hg) and decreased bradycardic response to higher pressure changes (more than 40 mm Hg) (p>0.05) indicating that neuronal circuitry of BST is an essential part of the baroreflex.. Conclusion: The present study indicated that the BST muscarinic receptors are involved in the cardiovascular regulation but are not involved in the modulation of baroreflex activity, although synapses within the BST have influence on the bradycardia baroreflex component.
Parva Nasimi, Mohammad Reza Tabandeh, Akbar Vahdati, Saeed Khatamsaz,
Volume 19, Issue 3 (September 2015)
Abstract

Introduction: Busulfan as a chemotherapeutic agent causes testicular germinal epithelium depletion and cytotoxicity in germ cells. The aim of this study was to assess antioxidant status, reactive oxygen species (ROS) generation and apoptosis-related genetic markers of adult male mouse sperm following busulfan treatment. Materials and Methods: Forty adult NMRI mice (30 ± 5 g) were divided into two groups. Control and busulfan treated group were administered with 100 &muL dimethyl sulfoxide and 3.2 mg/kg/day busulfan for 4 days, respectively. The superoxide dismutase and glutathione peroxidase assays were used for analyzing antioxidant status. Then, the levels of Bcl-2 family gene expression, lipid peroxidation and cytotoxicity were evaluated by Real-Time PCR, thiobarbituric and lactate dehydrogenase assays, respectively. Results: The results showed significant decrease on antioxidant status, increase on lipid peroxidation and lactate dehydrogenase in epididymal sperm and testis of busulfan treated mice in comparison with control (P< 0.05). Real Time PCR demonstrated significantly increased-Bax gene expression and decreased-Bcl-2 gene expression in epididymal sperm of treated group (P< 0.05). Conclusion: The high levels of lipid peroxidation and lactate dehydrogenase revealed increased-ROS and severe cytotoxicity in epididymal sperm and testis tissue following busulfan treatment at clinical dose. The oxidative stress and increased-ROS may induce Bcl-2 family gene expression-related apoptosis following busulfan therapy in normal cells.



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