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Showing 23 results for Testosterone


Volume 3, Issue 2 (11-1999)
Abstract

  Since the stress influences the biological processes, in the present study the effect of prenatal immobilization stress on the sexual activity of male rats was studied. Adult male and female Wistar rats (Pasteur Institute, Tehran) were copulated. After observing the vaginal plugs, pregnant rats were divided into control and two experimental groups. All groups were kept under the laboratory conditions, i.e. temperature adjusted at 22 ± 2 °C, a light and dark photoperiod of 13:11 hr and special food and water ad libitum. The pregnant control rats were under no stress, but experimental groups were put in the special adjustable restrainers from the beginning of the third week of pregnancy (14th day) which is the development period of the nervous system and the sexual dimorphic nucleus (SDN). One of the experimental groups was immobilized in the restrainer for two hours daily (from 9 to 11 a.m.) and the other for three hours daily (from 8 to 11 a.m.) for one week. The offspring were stayed with their mothers in the same cage for 40 days. Then male young rats (control and experimental groups with 9 rats in each of them) were put in the cages with experienced adult male and receptive female for one day to become familiar with the sexual experience. Then control and experimental male rats were placed individually in cages, each containing a receptive female and their sexual activities were observed. Measurement of serum hormones showed a significant decrease in testosterone level in experimental group compared to the control group that can be attributed to a decrease in the size of SDN and changes in the histology of testis. Also the level of estradiol and progesterone was increased in the experimental group. In addition, the histological study of testis showed a decrease in the number and diameter of seminiferous tubules in experimental groups compared to control. In conclusion, it seems that prenatal immobilization stress affects testicular histology in male rats and also decreases the serum level of testosterone and sexual activities.



Volume 9, Issue 2 (11-2005)
Abstract



Volume 10, Issue 0 (9-2006)
Abstract


Fatemeh Khakpay, Parvin Rostami, Aliasghar Pilevarian,
Volume 10, Issue 3 (11-2006)
Abstract

Introduction: Effective perception of fear signals is crucial for organism survival. When threated, the organism indicates defensive behaviors. Methods: Elevated plus–maze has high efficiency for measurement of fear behavior and is widly used for fear behavior determination. Increase in two parameters percent of openarm entries (%OAE) and percent of time spent in the open-arm (%OAT) in the elevated plus- maze were considered as the indexes of fear reduction effect. In this study, the effect of testosterone and naloxone on fear behavior of gonadectomized rats (GDX) was investigated. Also, the interaction of testosterone with naloxone was investigated. Results: Subcutaneous (SC) injections of different doses of testosterone (100, 200, 300, 450 μg /rat) increased the %OAE and %OAT in a dose dependent manner. Maximum response was obtained by 450 μg/rat dose of testosterone (P<0.001). SC injections of opioid receptors antagonist, naloxone 1, 2.5, 5, 7.5 mg / kg reduced %OAE and %OAT (P<0.01) . Conclusion: It may be concluded that testosterone decreases fear behavior and naloxone increased fear behavior. Also the results indicate that there is an interaction between testosteone and naloxone (P<0.001).
Shahrbanoo Oryan, Kazem Parivar, Masoumeh Asl-Rousta,
Volume 12, Issue 2 (8-2008)
Abstract

Introduction: Tamoxifen is a nonstroidal antiestrogen prescribed for treatment of breast cancer. The aim of this study was to investigate the effect of tamoxifen on testosterone level in the serum and sperm count in the epididymis of adult male Wistar rats. Methods: Three groups of rats received 200, 400 and 600 µg/kg body weight tamoxifen dissolved in solvent (60% ethanol in physiological solution) for 30 consecutive days. The sham group received the solvent and controls did not receive any drug or solvent. 1, 12 and 36 days after treatment, serum testosterone was measured by radioimmunoassay and sperm numbers in the epididymis were counted. Results: Results showed that testosterone concentration in the serum and sperm count in the epididymis significantly decreased in groups which received tamoxifen compared with the control group. The most profound effects were observed in the first samplings of the group which received 600 µg / kg tamoxifen. Conclusion: These findings indicate that tamoxifen decreases the fertilization ability in adult male rats in a dose dependent manner and this effect disappears after a period of time.
Farshad Moradpour, Nasser Naghdi, Yaghob Fathollahi,
Volume 12, Issue 3 (11-2008)
Abstract

Introduction: Sexhormones are one of most important factors in difference of learning and memory between tow sexes (male & female). High concentration of estrogen and androgen receptors and mechanism of testosterone and estrogen production in learning and memory center, especially in hippocampus show the role of this receptors, sexhormons and P450 enzymes in spatial memory. Consequently, in this study we evaluate effect of estradiol valerat and aromatase inhibitor (Anasterazole) on spatial learning and memory in Morris Water Maze. Materials and methods: Adult male rats were bilaterally connulated into CA1 region. After recovery period, control groups received DMSO 0/5µl and DMSO 0/5µl + DMSO 0/5µl. Different doses of estradiol valerat (EV) (1, 2.5, 5, 10 and 15µg / 0.5µl), anastrozole (An) (0.25, 0.5, 1µg / 0.5µl) and EV 15µg / 0.5µl +An 0.5µg / 0.5µl were microinjected all days before training. EV was injected 30-35 min before training and anastrozol was injected 25-30min before training. Result: Our results have showen EV 0.15µl / 0.5µl group increase escape latency and traveled distance. Also we have showen that anastrosole dose dependently decrease escape latency and traveled distance. Conclusion: We resulted that EV impaired acquisition of spatial learning and memory but anastrozole improved it. Anastrozole also couldn't be buffered EV – induced impairment.
Zeinab Sharifkhodaei, Nasser Naghdi, Shahrbanoo Oryan, Parichehr Yaghmaei,
Volume 12, Issue 4 (1-2009)
Abstract

Introduction: Neurohormones like testosterone and estradiol have an important role in learning and memory. The hippocampus is essentially involved in learning and memory, and is known to be a target for estradiol actions. Estrogen receptors (ERs) are highly expressed in CA1 of rat hippocampus, and mediate the effects of estrogen on learning and memory. Estradiol receptor belong to a family of transcription factors, the nuclear receptor superfamily, and has two subtypes ER and ER. The current research has been conducted to assess the effect of ER selective agonist, diarylpropionitrile (DPN), on passive avoidance of adult male rats, by using passive avoidance task.

Methods: Male adult rats were bilaterally cannulated into the CA1 area of hippocampus, and then received vehicle (dimethyl sulfoxide, DMSO) or DPN (0.2, 0.5, 1 micro-g/0.5 micro-l/side), 30 min before training on passive avoidance task.

Results: The results showed that pre-training intra-CA1 injections of DPN (0.5, 1 micro-g/0.5 micro-l/side), significantly decreased step-through latencies and increased time spent in dark on passive avoidance learning (P<0.01).

Conclusion: Our data suggest that intra-CA1 administration of DPN could impair learning and memory acquisition on passive avoidance task. 


Behnam Sedghi, Seddigheh Kahrizi, Hmidreza Zakeri, Kobra Omidfar, Mazaher Rahmani,
Volume 13, Issue 2 (8-2009)
Abstract

Evaluation of The Acute Hormonal Responses To Concentric, Eccentric And Concentric_ Eccentric Muscle Actions in Healthy Young Men Introduction: Resistance exercise is a potent stimulus for acute increase in the concentrations of circulating hormones such as Growth Hormone (GH) and Testosterone. Conventional resistance exercise is performed using sequential concentric (CON) and eccentric (ECC) contractions that is performed at a external constant load. The aim of this study, is determining hormonal and metabolic response to CON, ECC and CON-ECC muscle actions in order to develop appropriate resistance exercise protocols and equipment for both athletic populations and patients recovering from injury. In the present study, we have examined the endocrine system responses to six different upper and lower body exercise utilizing the CON, ECC and CON_ECC contractions. Methods: In this study, we examined acute hormonal responses of GH, TT & FT and cortisol of 10 young men who had experienced resistance exercises nonprofessionally. The exercise protocol included six resistance exercise which three of them were upper body and the other three were lower body exercises. The participants performed 3 contraction actions in 3 different days with at least one week interval and at the same time of the day. The exercise protocol included 4 sets in each exercise, every exercise was done 10 repetitions per set, and the resting time between exercises and sets was 90 seconds. CON and ECC actions performed at intensity of 80% CON 10-RM and 80 %( 120% CON 10-RM), respectively. Considering to the counterbalanced force design and on the calculations was done, the intensity of CON-ECC action was 88%CON 10-RM. Blood samples were collected before the exercise, immediately, 15, and 30 minutes after exercise session. Results: In the effect of CON, ECC and CON-ECC contraction actions GH increased, but after CON and CON-ECC trials increased significantly, only. In the effect of three actions, TT & FT increased significantly and following the three actions, cortisol decreased significantly. Conclusion: In despite of existence of significant increase in TT & FT acute response and significant decrease in cortisol response in the effect of CON, ECC and CON-ECC contraction actions, significant difference didn’t observe, that this issue demonstrating that the response of these hormones did not influence by type of contraction action. Also, between acute GH response related to these three actions, observed significant difference that demonstrating GH response depends on type of muscle action and degree of the motor units recruitment besides on intensity and volume of exercise. Key Words: Concentric and Eccentric Contractions, Acute hormone response, Growth Hormone, Total & Free Testosterone, Cortisol.
Akram Abdolahnejad, Ali Gol, Shahriar Dabiri,
Volume 13, Issue 3 (11-2009)
Abstract

Introduction: Diabetes mellitus has adverse effects on male sexual and reproductive functions in human and animals. Diabetes results in reduced fertility and libido. Medicinal plants have attracted much attention in controlling many diseases such as diabetes. In the present study, we aimed to investigate the effect of garlic juice on testicular damage. Methods: Forty male rats (250±20) were divided into 5 groups as follows: 1- Group normal (N) 2- Group Normal+Garlic (N+G) received garlic juice for 6 weeks. 3- Diabetic (D) received streptozotocin (STZ), 60mg/kg BW/i.p. 4- Group diabetic+garlic before (D+Gb) received garlic juice for 3 weeks before STZ injection and continued for more 3 weeks. 5- Group diabetic+garlic after (D+Ga) three days after STZ injection, they received garlic juice for 3 weeks. Garlic juice was given by gavage (1ml/100g BW). Number of leydig cells, testis weight, serum levels of testosterone and estradiol were assessed. Results: diabetic rats showed a marked decrease in the number of leydig cells, testis weight, serum levels of testosterone and estradiol. Garlic juice significantly increased the number of leydig cells, testis weight, serum levels of testosterone and estradiol in group 4 and 5 compared to group 3. The diabetic group receiving garlic before STZ injection showed more amelioration in complications than that receiving it after STZ injection. Conclusion: these results suggest that garlic juice supplementation could play both preventive and therapeutic role on testicular damage in diabetic rats.
Shoeib Rezaee, Sedighe Kahrizi, Mehdi Hedayati,
Volume 14, Issue 4 (1-2011)
Abstract

Introduction: This research was aimed at examining acute responses of hormones such as growth hormone (GH), total testosterone (TT), free testosterone (FT), free fatty acid (FFA) and cortisol to resistance (R), endurance (E) and endurance-resistance (ER) exercise. Methods: The participants of this study were 10 healthy young men. R protocol included bench press, lateral pull down, leg extension and leg curl. E protocol comprised of 30 minutes pedaling by cycle ergometer at 70% maximum heart rate. In the ER protocol, the participants first went through the E protocol and after an interval of 15 minutes they performed the R protocol. In the control group they did not do any exercise. Blood samples were collected before, immediately after and 15 minutes after exercise. Results: Results indicated that GH and FT were significantly increased after all 3 protocols (P<0.05). TT and FFA were significantly increased after R and E protocols, respectively (P<0.05). Comparison between groups indicated a significant difference between GH and FT. Cortisol was significantly decreased in the E and ER protocols and the control group (P<0.05) but it did not have a significant decrease in the R protocol. Conclusion: The E and ER protocols can increase GH with lower lactate and cortisol production, while the R protocol provides better anabolic environment for development and hypertrophy of muscle fiber because of higher stress, metabolic and hormonal responses. The increase of FFA after the E protocol was probably the cause of reduction of GH and TT secretion in the ER protocol.
Esmaeil Mohamadizadeh, Namdar Yousofvand, Maryam Kazemi,
Volume 15, Issue 2 (8-2011)
Abstract

Introduction: Thyroid hormones play an important role in the regulation of metabolism, maturity and reproduction. Thyroid dysfunction affects almost all endocrine glands such as pituitary and testis. The aim of this study was to investigate alterations of testosterone and luteinizing hormone (LH) serum levels as well as weights of thyroid gland and testes in methimazole (MMI)-induced hypothyroidism. Methods: Twenty-one adult male rats weighing 185 g were divided into 3 groups. The control group received drinking water, while treated groups received two doses of methimazole low dose (20 mg/dl in drinking water) and high dose (100 mg/dl in drinking water), for 42 days. At the end of the experiments, rats were anesthetized and sacrificed and serum samples were obtained. Serum levels of hormones were measured by radioimmunoassay. Weights of testes and thyroid gland were determined after sacrifice. Results: Results showed that the use of methimazole decreased serum levels of T4, T3, testosterone and LH as well as the weight of testes, while it increased the weight of thyroid gland compared to control group. These effects were more clear in the high dose group. Conclusion: This study suggests that MMI–induced hypothyroidism causes a significant decrease in serum levels of thyroid hormones, testosterone and LH. Hypothyroidism also decreases the weight of testes, while it increases the weight of thyroid in rat. These effects were dose-dependent.
Mohammad Reza Naghii, Mahmood Mofid, Ali Reza Asgari, Mahdi Hedayati, Maryam Sadat Daneshpour,
Volume 15, Issue 3 (10-2011)
Abstract

Introduction: Boron possesses widespread properties and is important for human and animal nutrition. Since Boron is rapidly bioavailable, the objective of the present study was to determine whether acute (hourly or daily), and weekly supplementationcould have any significant biological effects on the synthesis of steroids as well as inflammatory biomarkers. Methods: Eight male volunteers participated in experiments on three occasions (day 0, 1 and 7). On the first day (day 0), a blood sample was collected at 8.00 A. M, followed by ingestion of placebo. On the next day (supplementation- day 1), similar procedure was followed by ingestion of 10 mg of boron capsule. On both occasions samples of blood were collected every 2h for the next 6 h. Subjects consumed a capsule of 10 mg boron every day and on day 7, blood collection was carried out again at 8.00 A.M. Independent sample t-tests were used to evaluate the differences. Results: Plasma boron was significantly increasedfollowing hourly (P=0.002) and weekly (P=0.000) consumption of boron. After one week of supplementation, free testosterone levels were significantly increased (P<= 0.02) and estradiol concentrations were significantly decreased (P<= 0.01). Dihydrotestosterone (DHT), cortisol and Vitamin D showed slight non significant, increases. The ratios of free testosterone/testosterone (FT/T) (P<= 0.001), free testosterone/estradiol (FT/E2) (P<= 0.004) and testosterone/estradiol (T/E2) (P<= 0.009) were significantly increased. Also, all 3 inflammatory biomarkers were decreased after supplementation. Conclusion: Although there are previous studies that report a decrease in proinflammatory cytokines induced by boron consumption, to our knowledge, this is the first human study reporting an increase in plasma free testosterone concentrations following consumption of a boron supplement. This indicates a possible protective role against diseases of pathological conditions for this microelement.
Akram Hosseini, Abbas Ahmadi, Firouz Ghaderi Pakdel, Samad Zare,
Volume 15, Issue 3 (10-2011)
Abstract

Introduction: Cyclophosphamide (CP) is used for the treatment of various cancers. In spite of its therapeutic importance, a wide range of adverse effects such as reproductive toxicity has been observed following the administration of this drug in human and experimental animals. In the current study, we have investigated the adverse effects of CP on morphology and histology of testis rats. Methods: Twenty-one male Wistar rats were selected and randomly divided into 3 groups. CP was used at a dose of 6.1 mg/kg/day, (i.p.) for 50 days. At the end of the treatment, the histological and biochemical changes in testis, as well as sperm count and motility were assessed. Results: Testicular weight, sperm count and motility as well as serum testosterone concentration were significantly decreased whereas malondialdehyde (MDA) level was significantly increased in CP group compared with those in the control and sham groups. In addition, histological studies of testis structure showed that seminiferous tubules of testis were severely damaged in the CP group. CP increased the number of sloughing tubules and interstitial space, while it decreased seminiferous tubular diameter (STD), seminiferous epithelial height (SE), tubule differentiation index (TDI) and spermiation index (SPI). Conclusion: The results suggest that cyclophosphamide affect fertility parameters and cause testis atrophy after chronic treatment.
Gholamali Jelodar, Kobra Askari,
Volume 16, Issue 1 (4-2012)
Abstract

Introduction: PCOS is an important reproductive and endocrine disorder in women in the reproductive age. Present reports indicate that Vitex agnus-castus acts through hypothalamus-pituitary axis. This study was carried out to evaluate the effects of Vitex fruit hydroalcoholic extract on the alteration of sex hormones in a PCOS animal model. Methods: Following 14 days of monitoring of estrous cycles of rats, 32 animals with regular cycles were selected and randomly divided into four groups as follows: Control, treatment-control or sham (received 365 mg/kg Vitex extract for 30 days), PCO (received letrozole 1mg/kg for 28 days to induce PCOS) and PCT (treated with Vitex extract 365 mg/kg for 30 days after induction of PCOS). On the last day of experiments, estrous cycles of all animals were detected and blood was collected by heart puncture for evaluation of hormone levels. Results: The results showed that serum estradiol and progesterone levels decreased, while testosterone and DHEA levels increased following induction of PCO. No significant changes were observed in the hormone levels of the sham group compared to control. In the group treated with Vitex extract, a significant increase in serum levels of progesterone and a significant decrease in the serum testosterone levels were observed, however serum levels of estrogen and DHEA did not change significantly. Conclusion: Vitex agnus-castus fruit extract can improve serum levels of testosterone and progesterone in an animal model of PCOS, however it cannot change estrogen and DHEA levels significantly.
Maryam Rezaei, Azam Maghsoudi, Homayoun Khazali, Fariba Mahmoudi,
Volume 17, Issue 4 (1-2014)
Abstract

Introduction: Ghrelin and Orexin A exert inhibitory effects on gonadotropins secretion. Aromatase is a key enzyme in the steroidogenesis pathway which converts testosterone to the estradiol. Treatment of neonatal female rats with testosterone propionate (TP) alters gonadotropin secretion patterns in the adulthood. In the present study the effects of central injection of ghrelin or orexin A on the expression of aromatase gene in the ovaries of pubertal androgenized female rats. Methods: Forty two neonatal female rats were androgenized on the third day after birth by subcutaneous injection of 50μg TP and 6 neonatal female rats in one group received subcutaneous injection of olive oil as controls. After puberty, the animals in seven groups (n=6 in each group) received central injections of saline, different doses of ghrelin (2, 4 or 8μg) or Orexin A (2, 4 or 8μg). The ovaries were removed bilaterally and frozen. Aromatase gene expression levels was determined by semi quantitative RT-PCR. Results: The mRNA levels of aromatase (CYP19) increased significantly in the ovaries of the androgenized rats compared to the control group. Orexin A and ghrelin injections significantly decreased aromatase gene expression compared to the androgenized rats (P <0.05). Conclusion: Androgens may stimulate aromatase gene expression in the ovaries. Orexin A and ghrelin may exert inhibitory effects on reproductive axis partly via reducing the expression of genes involved in the steroidgenesis.
Negar Kayedi Bakhtiari, Hooman Eshagh Harooni, Ahmad Ali Moazedi, Mohammad Mohammadi,
Volume 18, Issue 2 (7-2014)
Abstract

Introduction: Previous studies have shown that zinc deficiency and castration could increase anxiety, while administration of zinc or testosterone has anxiolytic effects. This study examined the effect of zinc chloride administration on anxiety in gonadectomized male rats. Methods: For this purpose, adult male Wistar rats (weighing 200-250 g) were castrated. One month after surgery, different doses of zinc chloride (0, 5, 7.5 and 10 mg/kg IP) were administered 30 min before the elevated plus maze test. Time spent and the number of entries in open arms was recorded as measures of anxiety and the number of closed arm entries recorded as locomotor activity. Results: 1) Zinc chloride significantly decreased the time spent and the number of entries in the open arms in gonadectomized rats compared to the control group. 2) Zinc chloride administration could not decrease anxiety, even in the testosterone pretreated gonadectomized male rats. Conclusion: Our findings showed that zinc significantly increased anxiety in gonadectomized rats. Since our previous findings showed that zinc chloride decreased the anxiety level in intact male rats, it seems that zinc chloride effects on anxiety would change in relation to the presence or absence of the gonads and it might interact with androgenic system through an effect on the testis.
Fereshteh Ostovan, Ali Gol, Hakimeh Olomi,
Volume 18, Issue 3 (10-2014)
Abstract

Introduction: Diabetes drastically increases the risk of developing reproductive system disorders. The aim of this study was to investigate the effect of Citrullus Colocynthis pulp on testosterone and LH levels in streptozotocin-induced diabetic rats. Methods: Thirty-two male Wistar rats were divided into four groups as follows (n = 8 in each group): 1) normal, 2) pulp, 3) diabetic, and 4) treatment groups. Normal and diabetic groups orally received 2 ml normal saline for 2 weeks, while pulp and treatment groups orally received 30 mg/kg BW Citrullus Colocynthis pulp for 2 weeks. Diabetes was induced by a single intraperitoneal injection of streptozotocin (65 mg/kg BW). Results: The diabetic group showed a significant decrease in blood testosterone (p<0.001) and LH (P<0.01) levels as compared to the normal group. However, treatment group had a significant increase (p<0.01) in testosterone and LH levels as compared to the diabetic group. Conclusion: The data suggested that Citrullus colocynthis pulp administered at 30 mg/kg BW for 2 weeks may have beneficial effects on the damages in reproductive system, serum glucose impairment and loss of weight of testes caused by diabetes in rats through hypothalamus– pituitary–testis axis.
Fatemeh Aghaie, Homayoun Khazali, Mehdi Hedayati, Ali Akbarnejad,
Volume 20, Issue 4 (11-2016)
Abstract

Introduction: Polycystic ovary syndrome (PCOS) is one of the most common endocrinological pathologies in women during their reproductive years with ovulatory dysfunction, abdominal obesity, hyperandrogenism and insulin resistance. The aim of the present research was to evaluate the total antioxidant capacity (TAC), total oxidant status (TOS), free testosterone, ovarian morphology and estrous cyclicity in the estradiol valerate (EV)-induced PCOS rat model and the effect of treadmill and running wheel exercises on these parameters. Methods: Fifty female Wistar rats were randomly selected (220 ± 20 g). They had every 2 to 3 consecutive estrous cycles during 12 to 14 days. The first two groups were divided into control (n=10) and polycystic (n=40) that were induced PCOS by EV injection after 60 days. The polycystic groups were divided into three groups (n=10 in each group) PCOS, experiment group with treadmill exercise (running for 28 m/min at 60 min/day) and experiment group with running wheel exercise (running daily for 4 hours) for 8 weeks. Results: The PCOS rats had significantly higher testosterone, TOS and lower TAC than control. Eight weeks of treadmill and running wheel exercise significantly increased serum levels of TAC (just for treadmill exercise) and decreased level of TOS and T (just for treadmill exercise) in EV-induced PCOS rats compared to PCOS group. Ovarian morphology and estrous cycle was almost normalized in the PCOS exercise (treadmill and running wheel) groups. Conclusion: The present study demonstrate EV-induced PCOS in rats is associated with an increased oxidative stress and this increase can be returned to normal levels by exercise.


Vahid Azizi, Shahrbanoo Oryan, Homayoun Khazali, Abdolkarim Hosseini,
Volume 20, Issue 4 (11-2016)
Abstract

Introduction: Numerous studies have demonstrated that kisspeptin, a peptide from the KISS1 gene, plays an important role in regulating the secretion of gonadotropin releasing hormone (GnRH). Also, there is some evidence suggesting that kisspeptin can interact with other neuropeptides for the control of the reproductive axis. In the present study, we have investigated the effect of central administration of either kisspeptin or neuropeptide Y (NPY) or both on the mean plasma testosterone concentration in male rats. Methods: In this experimental study, 66 male Wistar rats were allocated into 11 groups (n=6 per group) receiving saline, kisspeptin (1 nmol), P234 (kisspeptin receptor antagonist, 1 nmol), NPY (2.3 nmol), BIBP3226 (NPY receptor antagonist, 7.8 nmol) or co-administration of them via intracerebroventricular (ICV) injection at 9:00-9:30 A.M. Blood samples were collected at 30 and 60 min following the injections for hormone assay. The serum testosterone concentration was measured using rat testosterone kit and the method of radioimmunoassay. Results: Kisspeptin or NPY injection significantly increased the mean serum testosterone concentration compared to saline at 30 and 60 min postinjection (P<0.001). The co-injection of kisspeptin+NPY considerably raised the mean serum testosterone concentration compared to NPY in both 30 and 60 min after the administration (P<0.001). This study indicates that P234 or BIBP3226 significantly attenuated (P<0.001) the testosterone increase after the kisspeptin injection compared to kisspeptin while a stimulatory increase effect was observed in the kisspeptin groups compared to either NPY or kisspeptin. Conclusion: Based upon the results, NPY may modulate the testosterone secretion indirectly via the kisspeptin signaling system.


Kameni Poumeni Mireille, Dzeufiet Djomeni Paul Desire, Bilanda Danielle Claude, Mengue Ngadena Yolande Sandrine, Ngoungouré Madeleine Chantal, Mballa Marguerite Francine, Mbolang Nguegan Lohik, Dimo Theophile, Kamtchouing Pierre,
Volume 21, Issue 1 (2-2017)
Abstract

Introduction: Nymphea lotus Linn (N. lotus) flowers are used empirically for the treatment of male sexual disorders in Cameroon west region. This study was aimed to evaluate the effects of the N. lotus on some parameters of male fertility in adult albino male rats. Methods: twenty four male rats were divided in 4 groups (n=6 each) and subjected to the following treatment: distilled water as vehicle control (10 ml/kg), testosterone as reference drug (5 mg/kg) and N. lotus flowers aqueous extract at the doses of 75 (NL75) and 150 mg/kg (NL150). Distilled water and aqueous extract of the plant were given once daily whereas testosterone was injected intramuscularly once a week. Treatment lasted for 55 days. Index of libido, sperm count, motility and viability were assessed. Relative weight and histology of some androgens dependent organs were analyzed. Results: Our results showed the treatment with NL75 during 55 days, increased markedly the index of libido (83.33%) in animals compared to control (50%). Both N. lotus treated groups showed a significant elevation (P<0.05) of the sperm count compared to testosterone treated animals. NL75 and NL150 also induced a significant increase of the sperm motility (P<0.001; P<0.01). NL75 induced a considerable increase in sperm viability (66.35% vs. 50.11%) compared to control. Further, NL75 also improved significantly the relative weight of testis (P<0.05), prostate (P<0.001) and penis (P<0.05) compared to control, suggesting anabolic effects of our plant. Conclusion: From these findings, it was concluded that N. lotus flowers have androgenic and reproductive properties justifying its empirical uses.



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