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Showing 2 results for Elevated Body Swing Test

Hashem Haghdoost-Yazdi, Mohadeseh Movahedi, Ayda Faraji, Mohamad Sophiabadi,
Volume 15, Issue 2 (8-2011)
Abstract

Introduction: β-carbolines (BCs) are heterocyclic indole alkaloids found in human body, grilled meat, alcoholic beverages and tobacco smoke. Based on the present reports, there are controversies about the role of these compounds in the pathophysiology of Parkinson' disease (PD). The aim of this study was to investigate the effect of norharman, a BC, in the prevention, exacerbation or creation of PD in 6-hydroxydopamine (6-OHDA) rat model. Methods: In the first part of this study, PD was established in rats by stereotaxic injection of 6-OHDA into the striatum. At the 2nd and 4th weeks post-surgery, apomorphine-induced rotational test was performed. Just before the surgery to the 4th week, after that rats received i.p. daily injections of norharman or its solvent at different doses. In the second part of the study, other groups of rats received daily intraperitoneal injections of norharman at different doses without prior injections of 6-OHDA. Fifteen and 30 days after the beginning of the injections, apomorphine-induced rotational and elevated body swing tests were performed. Results: In the 6-OHDA-induced Parkinsonism rats, daily injection of norharman at doses of 200 and 1000, but not 100 μg/kg, significantly increased apomorphine-induced rotations compared to the control group at the 4th week postsurgery. In the intact rats, daily injection of norharman at doses of 200, 500 and 1000 μg/kg could not produce any PD symptoms in the apomorphine-induced rotational and elevated body swing tests. Conclusion: Longtime exposure to BCs can exacerbate PD but it cannot create the disease.
Meysam Ghorbani, Parviz Shahabi, Abass Ebrahimi-Kalan, Hamid Soltani-Zangbar, Javad Mahmoudi, Soheila Bani, Behnaz Sadeghzadeh-Oskouei, Yusef Rafiee-Byraami, Omid Salimi,
Volume 22, Issue 4 (12-2018)
Abstract

Introduction: The use of standard rodent model, allows for the understanding of neuronal injury physiopathology and helping development of therapeutic strategies. Because of eliminating technical problems, we designed a modified impactor device with ability to induce different degrees according to kilodyne from very mild to very severe of spinal cord injury (SCI) and traumatic brain injury (TBI) models in rat. Methods: For standardization and determining of optimal performance of the device to induce varying injuries, 47 adult male Wistar rats were used, and 8 different forces were applied in spinal cord and brain tissues. Results: The hematoxylin and eosin and 2, 3, 5-triphenyltetrazolium chloride (TTC) results demonstrated that by increasing the level of forces, histological changes in the spinal cord and brain were significantly enhanced. Different injuries had significant effect on the Basso-Beattie-Brenham and elevated body swing test outcomes, and there were significant differences between groups in comparison with control group. Conclusion: Our results showed that the modified device could be valid to produce precise SCI and TBI models, goal to replicate SCI and TBI in humans as much as possible. However, it might be considered that aspects of SCI and TBI models are complicate and more examination is necessary.


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