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Showing 5 results for Doxorubicin

Mahboobeh Farokhpour, Khadijeh Karbalaie, Mahmood Etebari, Hamid Mirmohamad Sadeghi, Mohammad Hossein Nasr-Esfahani, Marzieh Nematolahi, Hossein Baharvand,
Volume 12, Issue 3 (11-2008)

Introduction: Doxorubicin is frequently used for treatment of several types of cancer. Doxorubicin cardiac toxicity has limited the use of this drug. Corticosteroids may prevent doxorubicin induced cardiotoxicity. Therefore the aim of this study was to evaluate mouse embryonic stem cells derived cardiomyocytes as a model to evaluate the effect of Doxorubicin and dexamethasone. Methods: Mouse embryonic stem cells derived cardiomyocytes were treated with different concentration of doxorubicin for 24 hours and the results were compared with control group. In order to exam effect of dexamethasone on cardiotoxicity, mouse embryonic stem cells derived cardiomyocytes were either exposed to 0.1, 1 or 10 µM dexamethasone 24 hours prior exposure to doxorubicin or expose to dexamethasone 24 hours before and during exposure to doxorubicin . Each group were compared with only doxorubicin or dexamethasone treated cells. Results: 5µM doxorubicin was selected as the lowest dose that ceased heart beats in more than 50% of Mouse embryonic stem cells derived cardiomyocytes. Results revealed that 10 µM dexamethasone for 24 hours before treatment with doxorubicin has protective effect on doxorubicin induced cardiotoxicity. Conclusion: The overall results obtained in this model are in accordance with previous literature. Thus suggesting that mouse embryonic stem cells derived cardiomyocytes is a suitable model for assessment of cardio toxic effects of drugs. Key words: Stem cell, Cardiomyocyte, Doxorubicin, Dexamethasone
Fatemeh Hamadani, Shahram Pourseyedi, Saeed Esmaeili Mahani,
Volume 18, Issue 2 (7-2014)

Abstract: Introduction: Cancer, a fatal disease, is the second leading cause of death reason exceeded only by cardiovascular disease. Breast cancer is common well known cancers in woman and is the second leading cause of death after lung cancer. In recent years researchers have focused on diet based on herbal medicine for the prevention and treatment of certain types of cancer. Methods: In this study, the cytotoxic properties of grape (Rishbaba) seed extract on MCF-7 human breast cancer cells were determined using MTT assay. Doxorubicin, an anti-cancer control drug, was used with the extract in combination therapy. Results: The data showed that incubation of cells with grape extract significantly reduced cell viability. Furthermore, concomitant treatment of cells with extract and anti-cancer drug produced a significant cytotoxic effect as compared to extract or drugs alone. Conclusion: Seeded grapes (vs seedless forms) probably have beneficial effects on the prevention and treatments of human breast cancer. In addition, their combination with chemotherapeutic agent doxorubicin may effectively induce cell death and could be be a potent modality to treat this type of cancer with reduced side effects.
Mohammad Rasoul Samandari-Bahraseman, Solmaz Sarhadi, Saeed Esmaeili-Mahani,
Volume 20, Issue 2 (5-2016)

Introduction: For thousands of years, plants have been used as the main source of drug worldwide. Recently, it has been found out that the plant Artemisia annua and especially its derivatives such as artemether have anticancer properties. Methods: In this study, the anticancer effect of artemether on MCF-7 breast cancer cell line was examined. MTT assay was used to assess the viability of cancer cells. Results: The data showed that artemether (100-300 nM) resulted in MCF-7 growth inhibition. Artemether plus vincristine or doxorubicin compared to each drug alone showed significant cytotoxic effects. Over 50 percent of cells were killed in combination of non-effective doses of artemether and doxorubicin (50 and 160 nM, respectively). Conclusion: Taken together, the combined therapy of artemether and anticancer drugs would be a promising strategy for breast cancer but the effectiveness of such combination therapy needs to be verified by experimental and clinical investigations.

Abbas Alimoradian, Hadi Ansarihadipour, Saeed Changizi-Ashtiyani, Ali Chehrei, Reza Talebi, Sadaf Davudian, Soheila Rostami,
Volume 22, Issue 1 (3-2018)

Introduction: The stress-oxidative is involved in doxorubicin (DOX)-induced cardiotoxicity. Due to the potential and previous reported for antioxidant properties of atorvastatin, omega-3, vitamin E and vitamin C, their efficacy to prevention of DOX-induced cardiotoxicity was investigated in this study. Methods: Fifty-six male rats were divided into 8 groups which received omega-3, atorvastatin, vitamin E, vitamin C, normal saline and dimethyl sulfoxide (DMSO) via gavage for 14 days then a single dose of DOX (20 mg/kg) was injected intraperitoneally except two last groups that received only normal saline or DMSO. The level of oxidative stress parameters like ferric reducing ability of plasma (FRAP) before and after DOX injection and malondialdehyde (MDA) of heart were estimated. Also the histopathologic assessments were done on heart sample at the end of experimental period. Results: The results showed that compared to other agents, omega-3 could emerge as the most protection against DOX. Its pretreatment led to one of the most FRAP changing percent meanwhile less MDA value and cardio pathologic indexes almost close to control groups compared to that of other agents (P<0.01). Conclusion: Omega-3 may have a promising protective effect against DOX-induced cardio toxicity.

Nazanin Entezari Heravi, Reza Mohebbati, Zohreh Naji Ebrahimi, Abolfazl Khajavi Rad, Mohammad Naser Shafei, Mohammad Soukhtanloo, Farimah Beheshti, Sara Hosseinian,
Volume 22, Issue 4 (12-2018)

Introduction: Nephropathy is defined as rational loss of renal function related with glomerulosclerosis and declining glomerular filtration rate. Inflammation and oxidative stress play a critical role in nephropathy. Plantago major has antioxidant effects. The aim of present study is the investigation of the effect of Plantago major hydro-alcoholic extract on the oxidative stress and renal function in kidney of rat. Methods: Rats were divided into five groups: control (Co), doxorubicin (DOX), doxorubicin+vitamin E (DOX+Vit E), 600mg/kg Plantago major (PM)+doxorubicin (PM600+DOX), 1200mg/kg Plantago major (PM)+doxorubicin (PM1200+DOX). DOX (5mg/kg, IV), Vit E and PM extract (600 and 1200mg/kg, PO) were administrated for 35 days. Finally, urine, blood samples and renal tissue were collected to measurement of redox markers, functional parameters and renal index percentage. Results: The renal superoxide dismutase (SOD) activity, total thiol and functional parameters significantly reduced and malondialdehyde (MDA) concentration increased in DOX group in comparison with control group. The renal SOD, catalase activities and total thiol content were significantly increased and MDA level decreased in PM treated groups along with DOX group in comparison with DOX group. The functional parameters significantly enhanced in treated groups with PM in comparison with the DOX group. The extract did not relive enhanced % renal index induced by DOX. Conclusion: Hydro-alcoholic extracts of PM, specially at its high dose led to an improvement in DOX-induced renal function and oxidative stress.

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