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Showing 19 results for Diabetes Mellitus


Volume 3, Issue 2 (11-1999)
Abstract

  Impaired wound healing is a well-documented complication in diabetes mellitus. With respect to the mechanism involved in impairment of wound healing in diabetic subjects and also the biological effects of fish oil and ω-3 fatty acids, in this study it was tried to examine whether fish oil-rich diet accelerates wound healing in diabetic rats or not? The adult male rats were divided into six groups. Group I (normal) consisted of normal rats, Group II (control) which received alloxan monohydrate, groups III (fish oil group) and IV (corn oil group or placebo) consisted of rats made diabetic by the alloxan (150 mg/kg s.c.) injection. Groups V and VI consisted of normal rats, which treated with fish oil or corn oil respectively. Groups III and V received 1.6 ml/day of fish oil, and groups IV and VI received 1.6 ml/day of corn oil in the pre- and post-operative days. All animals were wounded by a vertical 4 cm incision in the midline of dorsum. Wound surface area measured at days 0, 2, 5, 10, and 15 of post-operative period. Surface area of the wound in group III was less than group II (p<0.05) and group IV (p<0.05), in all of the post-operative days. Surface area of the wound in group III was the same as group V. Surface-area of the wound in group VI was more than group I (p<0.05). Percentage of wound healing in group III was more than groups II and IV (p<0.05). Healing time was less in group III compared to groups II and IV. These data suggest that fish oil (rich in ω-3 fatty acids), but not corn oil can accelerate wound healing.


Sohrab Esmailie Mahanie, Parvin Zareian, Reza Esmi Jahromie, Mohammad Khaksarie Hadad,
Volume 7, Issue 1 (4-2003)
Abstract

There are some evidences regarding anti-allergic and anti-inflammatory effects of Licorice common in traditional medicine and its usefulness in Diabetes mellitus and Diabetes insipidus and its stimulatory effect on admeocortical gland. This medicinal plant contains glycyrrhizin, sterogenic substances, cumarins, flavonoids, sterols, choline, asparagine and glaberin. Glycyrrhizin and glaberin have analgesic and anti- inflammatory effects. In this study, we evaluated the effect of licorice root extract on acute carrageenan-induced inflammation in the rat's hind paw as compared to anti- inflammatory effect of ibuprofen. For this purpose, adult male rats were divided into eight groups. Paw edema was induced by intraplantar injection of 0.1 ml of 0.5% carrageenan solution. Different doses of licorice root extract (50, l00, 200, 300 mg/kg, i.p.) and ibuprofen (12 mg/kg, i.p.) were given ten minutes before injection of carrageenan. Assessment of edema was performed by evaluation of volume change as determined by plethysmometry and extravasations of Evans blue dye as measured by spectrophotometry and changes in paw weight in test groups as compared to the control group. The results showed that the extract exerts a significant inhibitory effect on hind paw edema (volume) in a dose-dependent manner. In this respect, the maximum inhibition (54%) was achieved at a dose of 300 mg/kg of the extract and this dose was comparable to ibuprofen at a dose of 12 mg/kg. Meanwhile, the extract administration significantly reduced Evans blue content (100, 200 and 300 mg/kg) as compared to the control group. It could be concluded that licorice root extract is able to inhibit acute inflammatory response in the rat hind paw, and these effects are comparable to ibuprofen.
Mehrdad Habibi, Giti Torkaman, Babak Goosheh, Mehdi Hedayati,
Volume 13, Issue 1 (4-2009)
Abstract

Blood hypercoagulability or thrombogenicity can be one of the main causes of the cardiovascular diseases in young population without traditional pathologic risk factors such as diabetes mellitus, hypertension or hyperlipidemia. The effect of aerobic exercise on coagulation and fibrinolysis has been studied extensively Regarding to the effectivness of resistance exercises to increase functional ability of men, the purpose of this study was to examine the effects of the combined aerobic-resistance and aerobic exercise on the coagulation activity of healthy young men. 30 young healthy sedentary men (20±5 years old) randomly divided in 3 groups: 10 in the combined, 10 in the aerobic and 10 in nontraining matched control groups The training groups subjects were engaged in a program included, ten session, three times per week, with submaximal intensity and 24 min for every session. The combined group had 12 min resistance exercise, followed by a 12 min aerobic exercise on cycle ergometer although aerobic group had only 24 min aerobic exercise on cycle ergometer. The results revealed that PT decreased and PTT increased in training groups. Fibrinogen decreased significantly in both training groups. It was concluded that both submaximal aerobic-resistance and aerobic exercises, decrease coagulation system activity in young healthy sedentary men.
Akram Abdolahnejad, Ali Gol, Shahriar Dabiri,
Volume 13, Issue 3 (11-2009)
Abstract

Introduction: Diabetes mellitus has adverse effects on male sexual and reproductive functions in human and animals. Diabetes results in reduced fertility and libido. Medicinal plants have attracted much attention in controlling many diseases such as diabetes. In the present study, we aimed to investigate the effect of garlic juice on testicular damage. Methods: Forty male rats (250±20) were divided into 5 groups as follows: 1- Group normal (N) 2- Group Normal+Garlic (N+G) received garlic juice for 6 weeks. 3- Diabetic (D) received streptozotocin (STZ), 60mg/kg BW/i.p. 4- Group diabetic+garlic before (D+Gb) received garlic juice for 3 weeks before STZ injection and continued for more 3 weeks. 5- Group diabetic+garlic after (D+Ga) three days after STZ injection, they received garlic juice for 3 weeks. Garlic juice was given by gavage (1ml/100g BW). Number of leydig cells, testis weight, serum levels of testosterone and estradiol were assessed. Results: diabetic rats showed a marked decrease in the number of leydig cells, testis weight, serum levels of testosterone and estradiol. Garlic juice significantly increased the number of leydig cells, testis weight, serum levels of testosterone and estradiol in group 4 and 5 compared to group 3. The diabetic group receiving garlic before STZ injection showed more amelioration in complications than that receiving it after STZ injection. Conclusion: these results suggest that garlic juice supplementation could play both preventive and therapeutic role on testicular damage in diabetic rats.
Habibolah Saadat, Seyed Ali Ziai, Sima Nasri, Mina Sakenshaft, Atefe Ansarian,
Volume 13, Issue 4 (1-2010)
Abstract

Introduction: Angiotensin converting enzyme (ACE) is an exopeptidase that converts Angiotensin I to Angiotensin II. Angiotensin II is a potent vasoconstrictor and releases aldosterone, and have a critical role in hypertension. In this study, ACE insertion / deletion (I/D) polymorphism and ACE activity was determined in patients with coronary artery disease (CAD) and normal subjects. The correlation of these parameters with important CAD risk factors were also evaluated. Methods: 204 subjects were assigned to patients and normal groups based on their angiography results. Serum ACE activities were assayed by HPLC and I/D polymorphism were analyzed by PCR method. Important risk factors such as diabetes mellitus, hypertension, lipid profiles, ejection fraction, smoking and opium consumption were also recorded. Results: CAD was higher in DD genotype subjects (OR= 2.45 CI= 1.05-5.73), and ACE activity was about twice in DD compared to II genotypes. ACE activity was higher in hypertensives and diabetics in CAD group (p<0.001), but in the normal group it did not have any correlation with these risk factors. The rate of opium use was higher in ID and DD subjects. Conclusion: DD genotype is a risk factor for CAD and ACE activity is higher in this genotype. However, there is not any correlation between ACE activity and CAD.
Banafsheh Ranjbar, Iran Pouraboli, Mitra Mehrabani, Shahriar Dabiri, Abdolreza Javadi,
Volume 14, Issue 1 (3-2010)
Abstract

Introduction: Antioxidant agents have beneficial effects in diabetes mellitus. Daucus carota seeds extract has been shown to possess antioxidant activity. In this study, the effect of the methanolic extract of Daucus carota seeds on carbohydrate metabolism and morphology of pancreas was investigated in type I diabetic male rats. Methods: Type I diabetes mellitus was induced in male Wistar rats by injection of 70 mg/kg, i. p. of streptozotocin. Blood samples were collected from the eye cavernousa sinus, before and 5 days after injections for measurement of glucose and insulin. Diabetes was confirmed in rats that had FBS levels above 250 mg/dl. Diabetic rats were divided to 5 groups that received 100, 200 and 300 mg/kg of the extract, glibenclamide (600 μg/kg) and distilled water (0.5 ml) daily for 6 days by gastric gavage. After 6 days, they were sacrificed by decapitation and fasting blood samples were collected and serum levels of glucose and insulin were measured by spectrophotometric and ELISA methods, respectively, by using commercial kits. The pancreas of the rats were dissected out and fixed in 10% formaldehyde for histological studies. Results: Administration of all doses of Daucus carota seeds extract and glibenclamide for 6 days significantly decreased serum glucose levels, however, only 300 mg/kg of the extract as well as glibenclamide significantly increased insulin serum levels. Furthermore the extract and glybenclamide improved pancreas asinuses and islets as the number of islets significantly increased in rats receiving 100 mg/kg of the extract or glibenclamide. Conclusion: D. carota seeds extract has hypoglycemic effect by increasing insulin secretion and improvement of the pancreas.
Jalal Solati, Nastaran Soleimani,
Volume 14, Issue 2 (7-2010)
Abstract

Introduction: Herbal medicine and medical plants such as Ziziphus vulgaris L. are widely used for treatment of diseases such as diabetes mellitus. In the present study, we have investigated effects of alcoholic extracts of Z. vulgaris fruit on serum glucose, triglycerides, LDL, HDL and activities of aminotransferase enzymes in streptozocin (STZ)- induced diabetic adult male rats. Methods: Herbal material was dried, ground and then extracted with ethanol using Soxhlet apparatus. The combined extract was evaporated to dryness and the residue was dissolved in water and used for treatments. Adult male rats were rendered diabetic by a single i.p. injection of STZ (65 mg/kg). Normal and diabetic rats were daily treated with the extract dissolved in 0.5 ml distilled water (0.25, 0.5,1 and 1.5 g/kg) administered by oral gavage for 2 weeks. After 2 weeks of treatment, blood samples were collected from retro-orbital sinus of rats (Stone method) and serum level of glucose, insulin, triglycerides, LDL, HDL and activity of aminotransferase enzymes were measured using enzymatic methods. Results: Continuous supplementation of the extract at the doses of 0.5, 1 and 1.5 g/kg in diabetic rats resulted in a significant decrease of fasting blood glucose and triglyceride levels after 14 days compared to the control group. Levels of LDL, HDL and activities of serum aminotransaminase enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), were not significantly changed in the extract treated group with respect to the control. Conclusion: Obtained results showed that Z. vulgaris contain effective antidiabetic compounds and maybe useful for treatment of diabetes mellitus.
Sima Nasri, Zahra Hajializadh, Saeed Esmaeili-Mahani,
Volume 17, Issue 4 (1-2014)
Abstract

Introduction: Uncontrolled diabetes mellitus could lead to neuropathy in central and peripheral nerve tissues and one of its main signs can be hyperalgesia and motor coordination defect. Due to the blood glucose lowering effect of Thymus species and the presence of polyphenolic compounds with high antioxidant capacity, in this study the effect of Thymus caramanicus jalas extract was investigated on animal model of diabetes-induced neuropathy. Methods: In the present study development of hyperalgesia was examined by tail-flick and rota-rod tests, in streptozotocin-induced diabetic male wistar rats (subcutaneous injection). Animals were given Thymus caramanicus jalas extract (50, 100, 150 and 200 mg/kg) for 6 weeks. The levels of blood glucose were measured at the beginning and the end of the experimental period. Results: Blood glucose levels in diabetic animals which received Thymus extract at the doses of 150 and 100 mg/kg was reduced as compared to the pretreatment levels (p<0.05 and p<0.01, respectively). Untreated diabetic rats showed lower threshold in pain sensation and motor deficit compared with the control animals. However, tail-flick latency and ability to stay on rota-rod were significantly decreased in diabetic animals that received 100 mg/kg (p<0.01) and 150 mg/kg (p<0.001) of extract. Conclusion: The data show that Thymus caramanicus jalas extract has ability to reduce serum glucose levels and attenuate hyperalgesia and motor deficit induced by diabetes in rats. The mechanisms of this effect may be related to (at least in part) the attenuation of blood glucose and prevention of neural damage.
Fereshteh Ostovan, Hakimeh Olomi, Ali Gol,
Volume 17, Issue 4 (1-2014)
Abstract

Introduction: Diabetes mellitus is a metabolic disorder and it is estimated that its annual incidence rate will continue to increase in the future worldwide. Increased oxidant factors and decreased antioxidant defense are two of the factors resulting in diabetes. In the present study, we aimed to investigate Citrullus Colocynthis pulp effects on oxidant and antioxidant factors of liver in streptozotocin-induced diabetic rats. Methods: Thirty-two male rats were divided into four groups eight each: N (normal) group, N+C group, D (diabetic) group, and D+C group. Groups N and D received normal saline 2ml orally for 2 weeks and Groups N+C and D+C received 10mg/kg Citrullus Colocynthis pulp orally for 2 weeks. Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ) at 65 mg/kg. Results: Diabetic group had a significant increase in H2O2 (Hydrogen peroxide), MDA (malondialdehyde) and CAT (catalase) activity and a significant decrease in POD (peroxidase) activity in liver tissues compared to N and D groups. Group D+C had a significant decrease in H2O2, MDA, and CAT concentrations in liver tissues and significant increase in POD activity in liver tissues compared to D group. Conclusion: These results suggest that treatment of diabetic rats with Citrullus colocynthis pulp decreased oxidant stress and support antioxidant defense in liver STZ-Induced diabetic rats.
Maryam Arab Firouzjaei , Iraj Jafari Anarkoli , Mohammad Reza Jafari, Mehdi Eskandari , Mohsen Alipour,
Volume 17, Issue 4 (1-2014)
Abstract

Introduction: Memory impairment is one of the complications of diabetes which may accompany with changes in expression of apoptotic and antiapoptotic genes. The aim of the present study was the evaluation of intra-hippocampal injection of aminoguanidine (AG), as an antioxidant and inducible nitric oxide synthase inhibitor, on passive avoidance memory and Bcl-2 family genes expression in diabetic rats. Methods: Diabetes was induced in male rats using streptozotocin (STZ) (50 mg/kg, i.p). AG (10 and 90 μg/rat) was injected by intra-hippocampal implanted cannulae. Passive avoidance memory was assessed 7 weeks later. Then, animals were killed and hippocampus was removed. The expressions of Bcl-2, Bcl-xLand Bax mRNA were measured using semi-quantitative RT-PCR technique. Results: Diabetes caused significant impairment in passive avoidance memory. None of the AG doses improved the memory impairment. In diabetic rats, the levels of Bcl–2 and Bcl-xL were decreased in hippocampus while the expression of Bax, Bax/Bcl-2 and Bax/Bcl-xL was increased. In comparison to diabetic control group, AG treatment increased the levels of Bcl–2 and Bcl-xL but decreased Bax/Bcl–2 and Bax/Bcl-xL. Conclusion: Although AG was not associated with the significant improvement of memory but it modified the expression of the apoptosis involved genes in hippocampus of STZ-induced diabetic rats.
Simin Riahy, Mohammad Taghi Mohammadi, Vahid Sobhani,
Volume 18, Issue 1 (3-2014)
Abstract

Free radical can be defined as a molecule or molecular fragments containing unpaired electron in the outer orbital, which react with nearby molecules to get stability. There are two types of them in the body: oxygen free radicals and nitrogen free radicals. Our body has an antioxidant defense system which prevents accumulation of these radicals. There is a balance between free radical production and antioxidant defense system. Excessive free radical production or weak antioxidant system leads to oxidative or nitrosative stress. Diabetes mellitus is one of most important diseases that show cell injury due to oxidative and nitrosative stress in many tissues especially arteries. It causes atherosclerotic plaques in arteries by induction of inflammation, increasing the adhesive molecule expression, extravasation of circulating inflammatory cells, over-expression of some transcription factors, and fat deposition in the wall of arteries. Exercise is one of the main factors that influence production of free radicals and performance of antioxidant defense system. Although strenuous and acute exercise induces oxidative stress by increasing production of free radicals, but regular moderate exercise causes resistance against oxidative and nitrosative stress by potentiating antioxidant defense/repair systems. It appears that regular exercise accompanied by changes in life style is effective in reducing complications of diabetes, especially in prevention of atherosclerosis.
Omid Reza Tamtaji, Sayyed Alireza Talaei, Zahra Tamassoki, Mohsen Taghizadeh,
Volume 18, Issue 4 (1-2015)
Abstract

Introduction: Neuropathic pain is one of the common complications of diabetes mellitus which is caused by impairment in nerve conductivity. The role of flavonoid and polyphenol compounds in treatment of neuropathic pain has been revealed, and extract of onion contains significant amounts of these compounds. The aim of this study was to investigate the effect of alcoholic extract of onion on diabetic neuropathic pain in streptozotocin-diabetic rats. Methods: In this experimental study, 40 male Wistar rats were divided into 5 groups: normal control, diabetic control and groups receiving alcoholic extract of onion (125, 250 and 500 mg/kg/day). After injection of streptozotocin (55 mg/kg), the extract was administered for 3 weeks. At days 0, 7, 14 and 21 after injection of streptozotocin, assessment of neuropathic pain was performed by thermal allodynia, mechanical allodynia, hyperalgesia and formalin test. Results: Behavioral responses to thermal and mechanical stimuli in diabetic control rats showed significant reduction (P<0.05). Oral administration of alcoholic extract of onion at doses of 125 and 250 led to improvement in diabetic neuropathic pain in all 4 tests. However, dose of 500 mg didn’t improve neuropathic pain. Conclusion: Oral administration of alcoholic extract of Iranian red onion improves diabetic neuropathic pain in rats.
Sedigheh Khodsooz, Jamal Moshtaghian, Mehdi Eivani,
Volume 20, Issue 1 (2-2016)
Abstract

Introduction: Diabetes mellitus is a metabolic disorder of the endocrine system leading to increased blood glucose concentration in the patients. As a basic treatment for managing the blood glucose level, insulin or hypoglycemic medications are used but herbal medicines are more favored. The design of this research project was to study the therapeutic effect of hydroalcoholic extract of Melissa officinalis (HEMO) in diabetic rats.
Methods: Twenty-five Wistar male rats weighing 220±25 grams were distributed semi-randomly into five groups of five each. Group 1 and 2 was respectively the control and diabetic animals. Group 3, 4 and 5 were the diabetic animals treated with HEMO either at 20, 100 or 500 mg/Kg of body weight. To induce diabetic rat models, each animals received a single intraperitoneal injection of alloxan at the dose of 120 mg/Kg. All treatments with HEMO performed daily via gavage for a period of 4 weeks. Then, blood samples were collected from all animals to measure the blood glucose level, cholesterol, triglycerides, LDL and HDL.
Results: The results of this study indicated significant (P<0.05) decreases in blood sugar level, cholesterol, triglycerides and LDL in diabetic rats treated with HEMO. In addition, significant (P<0.05) increase in HDL level was observed in HEMO treated diabetic rats compared with the non-treated ones.
Conclusion: HEMO has significant effects on attenuating the blood sugar level, serum lipids and lipoproteins levels, whereas it improves the HDL level. These effect might be attributed to the antioxidant benefits of flavonoids which are present in HEMO.


Zahra Abbasi, Gholamali Jelodar, Saeed Nazifi,
Volume 21, Issue 1 (2-2017)
Abstract

Introduction: Walnut leaf contains large amounts of antioxidants, phenolic compounds and flavonoids and the beneficial effect of these compounds in the treatment of diabetes has been shown. This study investigated the effect of cyclohexane and ethanol extract of walnut leaf on activity of sorbitol dehydrogenase (SDH) enzyme. Methods: Fifty- six adult male Sprague-Dawley rats were divided into seven groups and treated for 30 days as follows: control and diabetic control (received sesame oil as vehicle), control cyclohexane & control ethanol (received 250 mg/kg body weight of cyclohexane and ethanol extracts respectively), diabetic cyclohexane 250 (received 250 mg/kg cyclohexane extract), and diabetic ethanol 150 & diabetic ethanol 250 (received 150 and 250 mg/kg ethanol extract respectively). Diabetes was induced by single injection of streptozotocin (60 mg/kg, ip). Body weight and blood glucose were recorded weekly and in the last day of treatment animals were sacrificed by whole blood collection directly from the heart. Activity of SDH was measured in the serum by ELISA method. Results: Oral administration of cyclohexane extract of walnut leaf at a dose of 250 mg/kg in the diabetic group improved blood glucose significantly compared to other diabetic groups. Administration of both extracts reduced activity of SDH compared to diabetic control group significantly. There was no significant difference of body weight between treatment groups and diabetic control group at the end of the treatment period. Conclusion: Cyclohexane extract of walnut leaf decreased blood glucose significantly, while both extracts reduced activity of SDH significantly in diabetic animals.


Mahnaz Rezaei-Kelishadi, Ali Ghasemi, Nasser Nabi Abdolyosefi, Setareh Zamani-Doabi, Majid Ramezani, Saeed Changizi-Ashtiyani, Azar Rahimi,
Volume 21, Issue 2 (5-2017)
Abstract

Introduction: Diabetes mellitus, as a chronic metabolic disease, is associated with a wide range of kidney and liver disorders. The goal of this study was to indicate the effects of selenium nanoparticles on the function of kidney and liver enzymes in stereptozocin induced diabetic rats. Methods: In this study, 35 male wistar rats were divided into five groups (n=7): control, diabetic control and three experimental groups receiving selenium nanoparticle solutions at doses of 0.1, 0.2 and 0.4 mg/kg/BW respectively. To induce diabetes in rats, a single dose of streptozocin (60 mg/kg/BW) was injected intraperitoneally. After the experimental period, blood samples were collected from all groups and the blood factors associated with the liver enzymes and kidney factors were measured and analyzed. Results: The results indicated that the levels of fasting blood sugar in all groups treated with selenium nanoparticles had a significant reduction compared to the diabetic group (P≤0.05). Aspartate amino transferase had a significant reduction at the maximum dose compared to the diabetic group (P≤ 0.05). Also a significant decrease in the level of albumin was seen in the group treated with selenium nanoparticle (P≤ 0.05). Selenium nanoparticle treatment made a significant decreases in the levels of urea and ceratinine at the maximum dose (P≤ 0.05). Conclusion: The consumption of selenium nanoparticles in proper dosages may have beneficial effects on diabetic complications by lowering blood sugar as well as reducing the increased levels of the liver enzymes and kidney factors, albumin and cratinine resulting in the better function of kidney and liver.


Parham Reisi, Fatemeh Sepahvand, Ghasem Zarei, Leila Kamali Dolatabadi, Shaghayegh Haghjooye Javanmard, Hojjatallah Alaei,
Volume 21, Issue 2 (5-2017)
Abstract

Introduction: Studies have indicated that diabetes mellitus impairs hippocampus. Diabetes increases the risk of depression and treatment with antidepressants may affect learning and memory. The aim of this study was to evaluate the effects of amitriptyline and fluoxetine on synaptic plasticity and TNF-α level in the hippocampus of streptozotocin-induced diabetic rats. Methods: Experimental groups were control, diabetes, diabetes-amitriptyline and diabetes-fluoxetine (n=8 for each experimental group). Three weeks after the induction of diabetes, the rats received treatment with amitriptyline (5 mg/kg) or fluoxetine (5 mg/kg) for 21 days. Long-term potentiation (LTP) in perforant path-dentate gyrus synapses was assessed (by 400 Hz tetanization) for investigating the effect of treatments on synaptic plasticity. Field excitatory post-synaptic potential indices were measured. Finally, TNF-α levels were measured in hippocampus by enzyme-linked immunosorbant assay. Results: Six weeks after the diabetes induction, LTP wasn’t different between the control and the diabetes groups and also no significant differences were observed between the diabetes and the diabetes-treated groups; however, amitriptyline and fluoxetine impaired LTP in diabetic rats and there was a significant difference between the control and the diabetes-treated groups. Comparing to the controls, TNF-α level was increased significantly (P<0.05) only in the diabetes-amitriptyline group. Conclusion: Results suggest that amitriptyline and fluoxetine intensify the destructive effects of diabetes on hippocampus and that TNF-α may act as a mediator for these changes; however, other factors may also be involved. Hence, treatment of diabetic patients with antidepressants must be done with extra care.


Samira Yazdanimehr, Mohammad Taghi Mohammadi,
Volume 22, Issue 1 (3-2018)
Abstract

Introduction: According to the powerful antioxidant effects of rosuvastatin, the present study aimed to examine the protective effects of rosuvastatin against oxidative damage of diabetic pancreas by potentiation of the antioxidant capacity in streptozotocin-induced diabetic rats. Methods: Experiment was performed in four groups of male Wistar rats (n=6 in each group): normal, diabetic and two treatment groups (normal and diabetic rats treated with rosuvastatin). Rats were made diabetic by a single intravenous injection of streptozotocin (40 mg/kg) at the beginning of study. Treatment groups received orally rosuvastatin at dose of 10 mg/kg/day. After eight weeks, the pancreas tissues were removed under deep anesthesia. After tissue homogenization, the contents of glutathione and malondialdehyde (MDA) as well as superoxide dismutase (SOD) activity were assessed by biochemical methods. Results: Blood glucose of diabetic rats was above 350 mg/dl. The MDA content of the homogenized pancreas significantly increased in diabetic rats by 92%. Diabetes also decreased the content of glutathione (32%) as well as SOD activity (68%) of pancreas tissues. Treatment with rosuvastatin noticeably decreased the MDA levels of diabetic pancreas (90%). Moreover, rosuvastatin significantly increased the glutathione content (21%) and SOD activity (67%) of pancreas tissues in treated diabetic rats. Conclusion: Our findings reveal that rosuvastatin is able to attenuate the uncontrolled hyperglycemia-induced oxidative damage of pancreas through potentiation of the antioxidant defense system.


František Ništiar, Agnesa Lukačínová, Oliver Rácz, Jaroslava Nováková, Eva Lovásová, Marek Brenišin, Simona Rusnáková,
Volume 22, Issue 1 (3-2018)
Abstract

Introduction: The aim of the present study was to evaluate the preventive effects of Flavin7 in prediabetic bio-breeding diabetes prone (BB-DP) rats. Methods: Foutthy rats were divided into 2 equal groups: group C (untreated control group) and group F7 with Flavin7 (natural dietary supplement F7 with bioflavonoids, 0.2 mg/l) in drinking water from 21st day after birth to 171st day of their life, respectively. Blood glucose, superoxide dismutase, glutathione peroxidase, catalase, total antioxidant capacity, glutathione, body weight, food intake, water intake and urine output were determined.  Results: The age of diabetes onset was significantly higher for group F7 compared to group C (P<0.05). The incidence of diabetes was lower in group F7 than in group C. Blood glucose at the diabetes onset was higher in group C than in F7 group (P<0.05). Decrease of antioxidant status parameters, at the treatment onset as well as immediately after its termination showed a drop in the F7 group firstly, but increased progressively later, until the end of the experiment. Conclusion: F7 delayed the development of diabetes in BB-DP rats and prevented its onset. The severity of diabetes mellitus was milder in rats treated with F7 than in control group.


Ramu Sathiya, Anita Murali, Jayaraman Anbu,
Volume 22, Issue 3 (9-2018)
Abstract

This review mainly deals with scientific data related to sporadic Alzheimer’s disease (AD) particularly related to diabetes mellitus (DM). AD is divided into sporadic AD and familial AD. It is known to be the most common cause of dementia. Sporadic form of AD results from multiple etiologic factors including metabolic, environmental and genetic factors. DM linked AD is known to be one of major challenges to health care system in these days. Both type 1 and type 2 DM is strongly related to cognitive impairment and known to be a major risk factor in the development of probable Alzheimer’s disease. In this review, the various mechanisms involved in the development of neuronal degeneration associated with chronic hyperglycaemia are discussed.


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