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Showing 8 results for Soleimani

Javad Araste, Ali Akbar Pourfathollah, Seyed Mohammad Moazzeni, Masood Soleimani,
Volume 7, Issue 2 (Fall and Winter 2003)

Therapeutic use of biological response modulators in combination with chemotherapeutic drugs may propose a more efficient way for cancer therapy with fewer side effects. However, the related mechanism has not been well understood. Γ-interferon is a modulator of biological responses that inhibits the growth of malignant cells and mediates their differentiation. In this investigation, T cell lymphoblastic leukemia-derived cell line (KE-37) was used for studying the synergistic effect of interferon and chemotherapeutic agents. For this purpose, this cell line was grown in tissue culture flasks and transferred to 96 well plates. Various doses of vincristine, methylprednisolone and dounorubicin were added to culture medium and the cells were grown at 370 °C incubator with 5% CO2. Then, 100 IU/ml of γ -interferon was added to cell culture at different times (at hours 0, 8, 36, and 60). The synergistic effect of γ-interferon and above-mentioned drugs was measured through MTT assay. Our results suggest that y-interferon could significantly increase the cytotoxic and/or cytostatic effects of vincristine, methylprednisolone, and tiounorubicin (p<0.05). In addition, this synergistic effect was more significant when γ-interferon was added 36 and 60 hours after addition of drugs (p<0.05).
Reza Moghadasali, Hossein Baharvand, Bahman Zeynali, Masoud Soleimani,
Volume 10, Issue 2 (Summer 2006)

Introduction: Embryonic Stem (ES) cells as pluripotent cells derived from the inner cell mass of blastula can differentiate to neural cells in vitro and this property is valuable in studies of neurogenesis and in the generation of donor cells for transplantation. In this regard, the propose of this research, was the study of the role of two important factors in the development of neural system, Fibroblast Growth Factor and Retinoic Acid were used in the study of mouse ESCs differentiation into neural cells. Methods: Royan B1 ESCs were used in this experiment. The formation of embryoid bodies (EBs) within 2days was the key indication of the differentiation process and then the treatment was carried out under the influence of different factors for example retinoic acid and fibroblast growth factor-2 within 4days in cell culture media in 6 groups and finally the EBs were transferred on poly-L-lysine coated dishes within 5 days to promotes the differentiation. Results: The Studying of β-tubulin III, as a marker of neural cells, in neural cells derived from the ESCs used immunocytochemistry method, and the results obtained from statistical analysis of the percentage of neural differentiated colonies, revealed that, retinoic acid is a strong inducer factor which caused the differentiation of ESCs into neural cells and under the influence of combination of fibroblast growth factor and retinoic acid, ESCs differentiated to the neural cells with longer and thicker outgrowth. Conclusion: This experiment showed that under the influence of fibroblast growth factor-2 and retinoic acid there is a possibility to generate an efficient and large numbers of neurons with longer and thicker outgrowth like motor neurons of spinal cord of posterior area which an suitable for transplantation in damaged spinal cord.
Mohamadreza Baghaban Eslaminejad, Farimah Salami, Malek Soleimani Mehranjani, Mohamad-Hossein Abnoosi, Poopak Eftekhari-Yazdi,
Volume 13, Issue 1 (Spring 2009)

Introduction: Previous investigations have indicated that the presence of BIO (6-Bromoindirubin-3-Oxime) in medium of some cell culture enhances the cell proliferation and viability. The aim of the present study was to investigate the BIO effects on in vitro expansion of rat marrow-derived mesenchymal stem cells (MSCs) culture. Methods: In the present experimental study, bone marrow cells from 7 rats were plated in the presence of 0.05, 0.01, 0.1, 1 and 1.5 µM of BIO and expanded through three successive subcultures. During the cultivation period, the cultures were statistically compared in terms of some indices of cell growth including the diameter and number of colonies, population doubling number (PDN) and the number of viable cells. Passaged-3 cells from all groups were examined whether or not they could differentiate into bone and adipose cells. Results: According to our results, the largest colonies were formed in the cultures with 0.1 and 1 µm BIO with diameter of respectively 1262.27±43.96 and 1335.71 ± 19.16 micrometer (P<0.05). These two groups were also superior in terms of the colonies numbers. During three successive passages, significantly more PDN was occurred in 0.1 and 1 µM BIO- treated cultures than the others (P<0.05). Additionally in these two BIO-treated cultures, the number of viable cells was significantly higher compared to other BIO-treated cultures as well as the control group (P<0.05). Alizarin red staining for bone and oil red for adipose cells indicated the differentiation potential of the cells in all studied groups Conclusion: Taken together it seems that the BIO presence in marrow cell cultures enhances the cell in vitro expansion and viability.
Jalal Solati, Nastaran Soleimani,
Volume 14, Issue 2 (Summer 2010)

Introduction: Herbal medicine and medical plants such as Ziziphus vulgaris L. are widely used for treatment of diseases such as diabetes mellitus. In the present study, we have investigated effects of alcoholic extracts of Z. vulgaris fruit on serum glucose, triglycerides, LDL, HDL and activities of aminotransferase enzymes in streptozocin (STZ)- induced diabetic adult male rats. Methods: Herbal material was dried, ground and then extracted with ethanol using Soxhlet apparatus. The combined extract was evaporated to dryness and the residue was dissolved in water and used for treatments. Adult male rats were rendered diabetic by a single i.p. injection of STZ (65 mg/kg). Normal and diabetic rats were daily treated with the extract dissolved in 0.5 ml distilled water (0.25, 0.5,1 and 1.5 g/kg) administered by oral gavage for 2 weeks. After 2 weeks of treatment, blood samples were collected from retro-orbital sinus of rats (Stone method) and serum level of glucose, insulin, triglycerides, LDL, HDL and activity of aminotransferase enzymes were measured using enzymatic methods. Results: Continuous supplementation of the extract at the doses of 0.5, 1 and 1.5 g/kg in diabetic rats resulted in a significant decrease of fasting blood glucose and triglyceride levels after 14 days compared to the control group. Levels of LDL, HDL and activities of serum aminotransaminase enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), were not significantly changed in the extract treated group with respect to the control. Conclusion: Obtained results showed that Z. vulgaris contain effective antidiabetic compounds and maybe useful for treatment of diabetes mellitus.
Mohammad Husein Abnosi, Malek Soleimani Mehranjani, Sayed Mohammad Ali Shariatzadeh, Majid Mahdiyeh Najafabadi, Laila Dehdehi,
Volume 15, Issue 3 (Fall 2011)

Introduction: In this study, the effect of para-nonylphenol as an environmental pollutant on viability, morphology and proliferation of bone marrow mesenchymal stem cells was investigated. Methods: Bone marrow mesenchymal stem cells of rat were treated with the 0.5, 1, 2.5, 3.5 and 5 μM of paranonylphenol for a period of 21 days, then the viability of the cells were estimated using trypan blue and MTT methods. After choosing the effective dose, the integration of the DNA was investigated using comet assay and agarose gel electrophoresis. Mechanisms of cell death were also investigated by TUNEL assay and presence of caspase activity. Results: The results showed that para-nonylphenol caused significant dose dependent reduction of viability and proliferation of the cells. Comet assay, agarose gel electrophoresis and TUNEL test showed that the DNA of the cells were damaged and broken after treatment with 0.5 and 2.5 μM of para-nonylphenol. In addition, activated caspase-3 was observed in the cytoplasm of treated cells. Conclusion: This study showed that a very low concentration of para-nonylphenol has drastic effects on bone marrow mesenchymal stem cells. This chemical is used in formulation of cosmetics and detergents and therfore may have detrimental effects on the viability and proliferation of stem cells.
Effat Soleimani, Manijhe Mokhtari Dizaji, Hajir Saberi, Shahram Shamshakimi, S. Raiesdana,
Volume 16, Issue 2 (Summer 2012)

Introduction:In this study, a non-invasive method based on consecutive ultrasonic image processing is introduced to assess time rate changes of the carotid artery wall displacement, velocity and acceleration in the longitudinal direction. The application of these parameters to discriminate healthy and atherosclerotic arteries was evaluated. Methods:Longitudinal displacement rate of common carotid artery wall was extracted with temporal resolution of 33 ms using a block-matching algorithm in three groups of subjects. The 3 groups consisted of 16 healthy men (group 1), as well as 16 men with less than 50% (group 2) and 16 subjects with more than 50% atherosclerotic stenosis in carotid artery (group 3). Differentiating the longitudinal displacement equation resulted in time rate changes of instantaneous velocity and acceleration during three cardiac cycles. Maximum and mean values of displacement and maximum and minimum values of velocity and acceleration were compared among the groups. Results:Maximum longitudinal displacement of the arterial wall was 0.438±0.116, 0.653±0.175 and 1.131±0.376 (mm) in groups 1, 2 and 3, respectively. Results of the statistical analysis (ANOVA), with confidence intervals of 95%, confirmed that there are significant differences (p<0.05) among longitudinal movement, velocity and acceleration of three groups of arteries. Conclusion:In the present study, time rate changes of kinematic parameters of the carotid artery wall motion in the longitudinal direction was evaluated, with temporal resolution of 33 ms. Healthy and atherosclerotic arteries were differentiated using these parameters. Our findings may help understanding the biomechanical behavior of the arteries.
Neda Soleimani, Elaheh Erami, Mehdi Abbasnejad, Shamsizadeh Ali, Azhdari-Zarmehri Hassan,
Volume 17, Issue 1 (Spring 2013)

Introduction: Despite significant progress in understanding pain control mechanism, there are numerous questions about central nervous mechanisms underlying stress-induced analgesia. The rostral ventromedial medulla (RVM) in the brainstem integrates a variety of functions, including pain modulation and pain perception. In the present study, we investigated the effect of temporary inactivation of RVM on stress-induced analgesia. Methods: This study was performed using adult male Wistar rats (200-250 gr). Swim stress was induced using a cylinder filled with water (50 cm height, 20±1°C) in which the rats were kept for 3 min. For induction of pain, 50 μL of 2% formalin was injected subcutaneously in the hind paw. For temporary inactivation of RVM, 0.5 μL of 2% lidocaine was injected into RVM. Results: Injection of lidocaine into RVM, before inducing swim stress, potentiated the anti-nociceptive effects of swim stress in phase 1 and phase 2A. In phase 2B swim stresses increased nociceptive scores of formalin test so administration of lidocaine into RVM inhibited the effect of swim stress. Conclusion: The result of this study demonstrated that temporal inactivation of RVM by lidocaine potentiated stress-induced analgesia.
Siamak Beheshti, Azam Soleimanipour,
Volume 21, Issue 1 (March 2017)

Introduction: Retinoid signaling has been argued to have favorable effects on Alzheimer's disease (AD). We studied the role of chronic intracerebroventricular (ICV) injection of all-trans retinoic acid (ATRA) on the amyloid-beta (Aβ) model of AD. Methods: Adult male rats weighing 260-330 g were divided into 12 groups of 8 each. Six groups of rats received ATRA (3nM, 30nM, 3μM, 0.3mM, 30mM/rat; ICV) or DMSO 1% (2μl/rat; ICV), bilaterally and in a chronic manner (6 times, twice a week). Forty eight hours following the last injection, memory performance was assessed using a passive avoidance paradigm. One group received Aβ (10μg/rat; ICV), bilaterally. The control group received DMSO 1% (2μl/rat; ICV). Twenty days later memory performance was assessed. Three groups of rats received Aβ (10μg/rat; ICV) and then ATRA (3nM or 30nM/rat; ICV) or DMSO 1%, chronically (6 times, twice a week). Another group received DMSO 1% (2μl/rat; ICV) and then, DMSO 1%, chronically (6 times, twice a week). Results: ATRA at doses 0.3mM and 30mM/rat impaired memory retrieval by decreasing step-through latency (STL) and increasing time spent in the dark compartment (TDC), significantly. However, moderate doses (3nM and 30nM/rat) did not change memory performance. ATRA (30nM/rat) increased STL and decreased TDC and NST in the Aβ-treated rats, significantly compared to the group received Aβ-DMSO 1%. Conclusion: The results propose a potential prophylactic effect of ATRA in the ICV Aβ model of AD and indicate the prominence of retinoic acid signaling as a target for AD prevention.

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