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Showing 7 results for Shabani

Mohammad Shabani, Saleh Zahedi Asl, Homa Manaheji,
Volume 10, Issue 1 (Spring 2006)

Introduction: Verapamil, a phenylalkylamin –type Ca2+ channel blocker, is widely used in the treatment of cardiovascular disorders especially as an antiaryhthmic and antiangina agent. Theoretically, calcium can influence thyroid function and there are evidences that Ca2+ channel blockers are able to interfere with thyroid function. In this study, the effects of chronic oral administration of verapamil on thyroid function of male Wistar rats were investigated. Methods: Study was performed on 5 groups of animals groups 1 to 3 were treated with verapamil at doses of 10, 20 and 50 mg /kg respectively for two months via oral tube. Sham group received only distilled water, while control group received neither verapamil nor distilled water. At the end of this period animals were anaesthetized, abdomen was opened and blood samples were obtained from abdominal aorta. The samples were centrifuged sera were separated and stored at – 20 ◦C until the time of the assays. Total triiodothyronine (TT3), total thyroxin (TT4), free triiodothyronine (FT3) and free thyroxin (FT4), T3 uptake levels were assayed by ELISA (DRG). Thyroid stimulating hormone (TSH) was determined by radioimmunoassay using DRG kits. Results: Total T4 level was significantly lower in sham (3.49 ± 0.1μg/dl) and verapamil dose 10 mg /kg (3.6±0.14) groups than in control group (4.5±0.34), while it was significantly higher in verapamil 50 mg /kg (4.24±0.2) group as compared to the sham group. Total T3 concentration in verapamil 20 mg /kg group (62±8.9ng/ dl) was decreased significantly compared to the control group (103.3±14). Free T3 and free T4 were significantly lower in sham group (p<0.005) compared to control group, while it was increased in verapamil groups of 20 and 50 mg /kg compared to sham group. Level of T3 up-take was decreased significantly (p<0.005) in sham (20.97±1.49%) and verapamil 20 (20.7±1.4) mg /kg compared to control group (27.6±1), while it was higher in verapamil 10 and 50 mg /kg groups than sham group. Thyroid stimulating hormone levels were similar in all groups. There were no significant differences in the T3/T4 ratio and body weights on first and last day of the groups compared to control group. Conclusion: It can be concluded that long term oral administration of verapamil doesn’t have inhibitory effect on thyroid function, however it can block adverse effect of handling stress on thyroid function. Therefore, from thyroid function point of view, the drug can be used safely for the duration of this study.
Mohammad Shabani, Masoud Haghani, Vahid Sheibani, Mahyar Janahmadi,
Volume 13, Issue 2 (Summer 2009)

Abstract* Introduction: Cannabis consumption during pregnancy may affect fetal growth, motor performance, memory and cognitive functions. Methods: Primiparous pregnant Wistar rats were randomly assigned to 4 treatment groups (6 rats per group) consisting of control and sham groups as well as 2 groups treated with Win. Win treated groups received daily s.c. injections of 0.5 or 1 mg/kg WIN suspended in 1% Tween 80 saline in a volume of 1 ml/kg from days 5 to 20 of pregnancy. Sham treated rats were given daily injections of 1% Tween 80 saline on the same days of pregnancy. Body weight, mortality, memory function and motor performance were assessed in the infants on the third, fifth and seventh weeks after birth. Righting responses in rat pups were also compared on the second day after birth. Results: Offsprings from Win (1mg/kg) treated rats exhibited a significant loss in the righting reflex on the 2nd day after birth, when compared to other groups. However, there was no statistically significant difference between groups regarding motor coordination assessed by rotarod test on the 3rd and 5th weeks after birth. Passive avoidance learning (PAL) test on 50-day-old rats showed that during the acquisition trials, approach latencies were not significantly different among all groups. However, in retention trials, which were performed 24 h and 7 days later, the avoidance latencies of rats exposed to 0.5 mg/Kg Win were significantly shorter than those of the control and sham animals. Win consumption during pregnancy did not induce significant changes in the body weight of pregnant rats on the first day and three weeks after pregnancy. Litter size (number of pups per delivery) was significantly reduced in 1 mg/kg Wintreated compared to 0.5 mg/kg Win-treated, sham treated and control groups (p<0. 001). The length of pregnancy in 1 mg/kg Win-exposed rats was also significantly shorter compared to control (p<0.001), 0.5 mg/kg win-treated (p<0.01), and sham (p<0.0001) rats. Conclusion: These findings suggest that prenatal exposure to Win, a cannabinoid agonist, probably induces a longterm alteration of the endocannabinoid system, which in turn affects learning and motor coordination ability. Keywords: Cannabinoids, Maternal Consumption, motor performance, memory.
Ahdiye Shojaei, Mohammad Shabani, Asghar Pilevarian, Shahrnaz Parsania, Moazameh Razavinasab ,
Volume 16, Issue 2 (Summer 2012)

Introduction: For some cancer survivors chemotherapy treatment is associated with lasting motor and cognitive impairments, long after treatment cessation. Cisplatin as an anti-neoplastic agent is extremely toxic and can cause severe tissue damage. In the present study, we elucidated alteration in performance of hippocampus- and cerebellum-related behaviors following acute cisplatin treatment in male and female rats. Methods:Male and female wistar rats (120) were divided randomly into eight (two controls [saline] and 6 cisplatin) groups. Cerebellum- and hippocampus-related behavioral dysfunction in cisplatin-treated (5, 10 and 15 mg/kg/week for 1 week) rats were analyzed using hippocampus and cerebellum- dependent function tasks (Morris Water Maze, Shuttle box, Rotarod and Open field). Results:Exposure to cisplatin impaired motor coordination in male and female rats in all doses. In open field test, the rearing frequency, total distance moved and velocity of both males and females were dramatically affected by exposure to cisplatin. In Morris water maze test, male and female rats that were trained one week after cisplatin injection showed significant memory deficits compared to the saline-treated rats. Conclusion:Hippocampal and cerebellum functions of male and female rats were profoundly affected by exposure to cisplatin. No sex-difference was observed in the most measured variables.
Mohammad Shabani, Taybeh Khezrifard, Mobin Aghapour, Shahrnaz Parsania,
Volume 16, Issue 4 (Winter 2013)

Abstract Introduction: Mobile phones create high-frequency electromagnetic fields around them when in use. The close proximity of mobile phones to the user’s head leads to the absorption of part of the electromagnetic waves into the head and the brain. Methods: We have elucidated the alteration in cognitive function of the male and female offspring rats, which were exposed to chronic mobile phone exposure during their gestational period using Shuttle box and Morris Water Maze (~20 days). Results: The results of the passive avoidance and Morris Water Maze assessment of learning and memory performance showed a decrease in learning and memory in rats exposed to mobile phone. Exposure to EMF increased the shock number (Shuttle box test) and distance (Morris Water Maze test) compared to control groups (P<0.05). When the testing was performed 1 and 7 days after the shock experience (28-30 days post- mobile phone exposure), the step-through latency was significantly decreased in male and female mobile phone-exposed rats compared to the control rats (P<0.01). Results from the probe test were measured as mean percentage [%] time spent in correct quadrant showed that mobile phone exposure significantly decreased the percent time spent in the correct quadrant compared to control (p<0.05). Conclusion: Our results suggest that, mobile phone is able to induce a serious disruption in learning and memory and these observed deficits would suggest that we should limit the usage of these instruments during pregnancy.
Mohammad Shabani, Peyman Yaghmaei, Hossein Mohtashamipour, Moazamehosadat Razavinasab, Masoud Nazeri,
Volume 18, Issue 2 ( Summer 2014)

Introduction: Sub-chronic swim stress is known to induce a prolonged hyperalgesia, which is mediated through NMDA and opioid systems. Nitric oxide is a soluble gas, which acts as a retrograde messenger that modulates the release of mentioned neurotransmitters. It is also involved in nociception and memory. The aim of this study was to evaluate the role of NO pathway in nociception and memory disruption induced by sub-chronic swim stress. Methods: Three sessions forced swimming stress protocols were applied to rats. Before each swimming session, pretreatment with L-NAME (10 mg/kg, i.p.), L-Arginine (10 mg/kg, i.p.) or saline was made. Passive avoidance learning, nociception and anxiety-like behavior were evaluated 24 hours after last swim stress session. Results: Results showed that step through latency was decreased after swim stress and it could be inhibited by pretreatment with L-NAME. Swim stress increased anxiety-like behavior in the open field test, which could be inhibited by pretreatment with L-NAME and L-Arginine. Reduced thermal threshold was observed in the nociceptive measurement after swim stress. Pretreatment with L-NAME could reverse this reduced threshold. Conclusion: The results of this study indicate that sub-chronic swim stress impairs nociception and passive avoidance learning. It seems that NO pathway have a modulatory role in these alterations.
Mohammad Reza Lashkarizadeh, Mohammad Garshasbi, Shahriar Dabiri, Mahdieh Lashkarizadeh, Mohammad Shabani,
Volume 19, Issue 3 (September 2015)

Introduction: Opium addiction can change immune response to Types of stress such as injury or trauma due to alterations in the in secretion status of cytokines in the body. In this study, effects of opium addiction on wound healing and post-operative adhesion bands were assessed after laparotomy. Materials and Methods: Male rats (n=20) were randomly divided into opium addicted (documented with Naloxone test) and control group. Three weeks after surgery, site of abdominal incision was excised elliptically and sent for wound healing grading assessment by pathologist and an intra-abdominal adhesion band assessment was done. The concentrations of three cytokines (TNF , IFN and IL10) were also measured before, immediately after surgery and 24 hour after surgery. Results: Post-operative intra-abdominal adhesion was decreased in opium addicted group in comparison to control group (p value = 0.014). No statistically significant difference was found in the wound healing phase in two groups (P value = 0.057). Our findings showed that serum level of TNF , IFN and IL10 in two groups measured in all phases of examination (before surgery, within 30-60 min after surgery and 24h after surgery), were not statistically different/significant (p>0.05). Conclusion: Since opium addiction can decrease post-operative intra-abdominal adhesions in rats, they may be susceptible to increased inflammation and these effects may be due to decreased post-operative pain.

Bentolhoda AmirShabani, Mohammad Rostampoor, Parvin Babaei,
Volume 21, Issue 3 (September 2017)

Introduction: Protein kinase M zeta (PKMζ) is assumed to be actively involved in retainig long-term potentiation. The goal of this study was to investigate the role of PKMζ in basolateral amygdala (BLA) upon acquisition, consolidation, retention and retrieval of memory using a specific inhibitor of PKMζ. Methods: Sixty male wistar rats underwent stereotaxic surgery and were cannulated bilaterally at the BLA nucleus. Then animals were divided into 4 groups of receiving BLA microinjection of zeta inhibitory peptide (ZIP) in different time courses: 30 min before and after training, 30 min before the testing (on the day after the learning) and 30 min after testing (but testing 10 days later). Memory was assessed using step through passive avoidance. Results: ZIP infusion in BLA had no significant change on acquisition (P=0.06), however significantly impaired consolidation, retrieval and maintenance of passive avoidance memory (P=0.012). Conclusion: Findings indicate that PKMζ activity in the BLA plays an important role in retaining amygdala dependent avoidance memory interfering the process of consolidation, retrieval and maintenance of learned task.

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