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Showing 5 results for Salami

Mahmood Salami Zavareh, Yaghub Fathollahi, Hossein Esteky, Fereshteh Motamedi, Nafiseh Atapour,
Volume 2, Issue 2 (Fall and Winter 1998)

Mahmood Salami, Yaghub Fathollahi, Fereshteh Motamedi, Hossein Esteky,
Volume 3, Issue 1 (Spring and Summer 1999)

  The effectiveness of θ pattern primed-bursts (PBs) on development of primed-burst (PB) potentiation was investigated in layer II/III of the adult rat visual cortex in vitro. Experiments were carried out in the visual cortical slices. Population excitatory post-synaptic potentials (pEPSPs) were evoked in layer II/III by stimulation of either white mater or layer IV. To induce long-term potentiation (LTP), eight episodes of PBs were delivered at 0.1 Hz. Regardless of stimulation site, field potential recorded in layer II/III consisted of two components: a short latency and high amplitude response called pEPSP1, and a long latency and low amplitude response called pEPSP2. The incidence of LTP produced by PBs of layer IV was higher than that of the white mater tetanization. In contrast, PBs of both layer IV and white mater reliably induced LTP of pEPSP2 in layer II/III. It is concluded that PBs, as a type of activity pattern, of either white mater or layer IV can gain access to the modifiable synapses that are related to pEPSP2 in layer II/III, but accessibility of the modifiable synapses that are related to pEPSP1 depends on tetanization site. Relevancy of the results to the plasticity gate hypothesis is also discussed.


Mahmood Salami, Yagoob Fathollahi,
Volume 6, Issue 1 (Spring and Summer 2002)

In this study, involvement ofvoltage-dependent calcium channels in LTP of responses of rat visual cortex slices was analyzed. Field potentials including EPSP1 and EPSP2 from layers II/III were recorded through stimulation of layer IV. Whereas nifedipine, a L-type calcium channel blocker (L-VDCC), did not considerably affect the LTP of responses, but Ni2+, a relatively selective blocker of T-type calcium channels (T-VDCC) decreased potentiation of EPSP1 and partly blocked that of EPSP2. The effect of visual experience on the function of channels was also evaluated. The results showed that T-VDCCs mediate a transient augmentation of EPSP1, while contribute to a stable LTP of EPSP2. Whereas sensory experience influences the occurrence of LTP in field potentials, it seems to be ineffective on the role of calcium channels in synaptic plasticity. It is concluded that the T-VDCCs alone by allowing calcium influx into neurons or by sharing NMDA receptors are involved in the synaptic plasticity.
Ghola Ali Hamidi, Homa Manaheji, Mahyar Janahmadi, Mahmood Salami,
Volume 10, Issue 1 (Spring 2006)

Introduction: Neuropathic pain syndromes are changes resulting from damage to neuronal pathways that are characterized by spontaneous burning sensation with accompanying allodynia and hyperalgesia. Since the treatments of neuropathic pain are poorly understood and existing treatments are often ineffective, it is important to increase our understanding of the neuropathic pain states in order to identify strategies for the development of effective therapies. The purpose of this study was to investigate the involvement and pre-emptive treatment of morphine and / or NMDA receptor antagonist MK-801, and co-administration of both drugs on behavioural responses in an experimental model of neuropathic pain (CCI). Methods: Experiments were performed on six groups (n=8) of male Sprague-Dawley rats (230-280g). In the groups that received drugs, two groups were injected with MK-801 (0.3 mg/kg, 20 min before, and 6 h after the operation) or morphine (8 mg/kg, 30 min prior to the operation). Another group received both drugs with the same doses and protocols. Finally, one group received normal saline in same volumes. The animals were tested for allodynia and hyperalgesia reactions at 0, 3, 7, 14, 21 and 28 days after CCI of the sciatic nerve. Results: Our data revealed that the CCI produces mechanical and cold allodynia and a hypersensitivity to noxious stimulations. MK-801 and morphine produced only a slight cold anti-allodynic response. On the other hand, co-injection of morphine and MK-801 markedly reduced cold allodynia at the days 7 (P<0.01), 14 (P<0.05) and 21 (P<0.05) when compared with the saline group. However, there was slight alleviation of the mechano allodynia, and, heat- and mechano-hyperalgesia. Results demonstrate that the CCI model importantly influences the behavioural responses to both the thermal and mechanical stimulations. Conclusion: We conclude that co-administration of both drugs can be more effective than MK-801 and morphine administered alone in the induced neuropathic pain.
Mohamadreza Baghaban Eslaminejad, Farimah Salami, Malek Soleimani Mehranjani, Mohamad-Hossein Abnoosi, Poopak Eftekhari-Yazdi,
Volume 13, Issue 1 (Spring 2009)

Introduction: Previous investigations have indicated that the presence of BIO (6-Bromoindirubin-3-Oxime) in medium of some cell culture enhances the cell proliferation and viability. The aim of the present study was to investigate the BIO effects on in vitro expansion of rat marrow-derived mesenchymal stem cells (MSCs) culture. Methods: In the present experimental study, bone marrow cells from 7 rats were plated in the presence of 0.05, 0.01, 0.1, 1 and 1.5 µM of BIO and expanded through three successive subcultures. During the cultivation period, the cultures were statistically compared in terms of some indices of cell growth including the diameter and number of colonies, population doubling number (PDN) and the number of viable cells. Passaged-3 cells from all groups were examined whether or not they could differentiate into bone and adipose cells. Results: According to our results, the largest colonies were formed in the cultures with 0.1 and 1 µm BIO with diameter of respectively 1262.27±43.96 and 1335.71 ± 19.16 micrometer (P<0.05). These two groups were also superior in terms of the colonies numbers. During three successive passages, significantly more PDN was occurred in 0.1 and 1 µM BIO- treated cultures than the others (P<0.05). Additionally in these two BIO-treated cultures, the number of viable cells was significantly higher compared to other BIO-treated cultures as well as the control group (P<0.05). Alizarin red staining for bone and oil red for adipose cells indicated the differentiation potential of the cells in all studied groups Conclusion: Taken together it seems that the BIO presence in marrow cell cultures enhances the cell in vitro expansion and viability.

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