Search published articles


Showing 5 results for Rostami

Fatemeh Khakpay, Parvin Rostami, Aliasghar Pilevarian,
Volume 10, Issue 3 (Fall 2006)
Abstract

Introduction: Effective perception of fear signals is crucial for organism survival. When threated, the organism indicates defensive behaviors. Methods: Elevated plus–maze has high efficiency for measurement of fear behavior and is widly used for fear behavior determination. Increase in two parameters percent of openarm entries (%OAE) and percent of time spent in the open-arm (%OAT) in the elevated plus- maze were considered as the indexes of fear reduction effect. In this study, the effect of testosterone and naloxone on fear behavior of gonadectomized rats (GDX) was investigated. Also, the interaction of testosterone with naloxone was investigated. Results: Subcutaneous (SC) injections of different doses of testosterone (100, 200, 300, 450 μg /rat) increased the %OAE and %OAT in a dose dependent manner. Maximum response was obtained by 450 μg/rat dose of testosterone (P<0.001). SC injections of opioid receptors antagonist, naloxone 1, 2.5, 5, 7.5 mg / kg reduced %OAE and %OAT (P<0.01) . Conclusion: It may be concluded that testosterone decreases fear behavior and naloxone increased fear behavior. Also the results indicate that there is an interaction between testosteone and naloxone (P<0.001).
Hatam Ahmadi, Parvin Rostami,
Volume 12, Issue 1 (Spring 2008)
Abstract

Introduction: Stress has many effects on the development of systems and organs in the fetal period, and these effects appear after birth. Since hemopoietic system is susceptible to stress, effects of restraint stress were studied in offspring of pregnant rats. Methods: Pregnant rats were divided into one control and three stress groups. The control group did not receive any stress during the gestational period. Stress groups 1, 2 and 3 were subjected to restraint stress from 8 to 21, 8 to 17, and 17 to 21 days of gestation, respectively. At the age of 60 days, the blood samples were taken from the male offspring rats. Results: The results in the offsprings were as follows: a) Restraint stress markedly decreased the total number of white blood cells in offsprings of groups 1 and 3 . The percentage of granulocytes decreased and lymphocytes increased significantly in these groups. b) The number of red blood cells increased significantly in groups 1 and 2 compared with the control group. c) The number of platelets increased in group 1, although their hemoglobin decreased significantly. d) As for the index of RBC, the prenatal stress had an effect on MCV, MCH and MCHC in all groups. Conclusion: Our results showed that prenatal restraint stress causes long lasting changes in the blood parameters after birth. These data prove that restraint stress alters the function of immune and hemopoietic systems.
Parichehr Hassanzadeh, Elham Arbabi, Fatemeh Rostami, Fatemeh Atyabi, Rassoul Dinarvand,
Volume 19, Issue 3 (September 2015)
Abstract

Introduction: Carbon nanotubes (CNTs) have shown enormous potential in neuroscience. Nerve growth factor (NGF)-CNTs complex promotes the neuronal growth, however, the underlying mechanism(s) have remained elusive. Based on the interplay between NGF and the endocannabinoid system, involvement of the neuroprotective endocannabinoid, 2-arachidonoyl glycerol (2-AG), was investigated in the mechanism of action of NGF. Materials and Methods: Multi-walled CNTs (MWCNTs)-NGF complex was prepared using amino-functionalized COOH-MWCNTs. MWCNTs were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). In vitro cytotoxicity was evaluated by MTT assay. Following three times daily intracerebroventricular injections of NGF solution (2, 5, and 10 &mug), and 5, 10, and 20 &mug of acid- or amine-modified MWCNTs, or MWCNTs-NGF complex for either one or 7 days, 2-AG contents were quantified in the frontal cortex and hippocampus of rats by isotope-dilution liquid chromatography/mass spectrometry. Results: FTIR confirmed the amino-functionalization of COOH-MWCNTs and NGF immobilization on the aminated MWCNTs. Aminated MWCNTs and MWCNTs-NGF complex showed less cytotoxicity than COOH-MWCNTs (P<0.05, P<0.01, and P<0.01). Chronic, but not acute, administration of MWCNTs-NGF complex and NGF solution at the highest dose tested led to the elevation of 2-AG at 1 h from the last injection (P<0.01 and P<0.001). 2-AG enhancement induced by MWCNTs-NGF complex lasted for up to 5 and 12 h post-injection (P<0.01 and P<0.001). 2-AG contents remained at the baseline level in the sham and groups receiving vehicle, acid- or amine-modified MWCNTs (P>0.05). Conclusion: Functionalized MWCNTs-NGF complex induces a long-lasting increase of brain 2-AG content indicating the efficiency of this nanostructure to provide a sustained concentration of NGF. Implication of 2-AG in the mechanism of action of NGF might be of great therapeutic significance in the neurological disorders.


Parichehr Hassanzadeh, Elham Arbabi, Fatemeh Rostami, Fatemeh Atyabi, Rassoul Dinarvand,
Volume 21, Issue 4 (December 2017)
Abstract

Introduction: Treatment of lung diseases is one of the major healthcare challenges. Ferulic acid (FA), a phenolic compound with well-established antioxidant and anti-inflammatory properties, has shown promising therapeutic potential against the pulmonary disorders; however, low bioavailability may negatively affect its efficiency. This, prompted us to incorporate FA into the nanostructured lipid carriers (FA-NLCs) and evaluate the toxicity of this nanoformulation in human lung adenocarcinoma cell line (A549) and its suitability for pulmonary drug delivery. Methods: FA-NLCs were prepared by high-pressure homogenization followed by assessment of the physicochemical properties of nanoparticles, in vitro release profile, aerosol characteristics, in vitro cytotoxicity, pharmacokinetic parameters and lung deposition of the nanoparticles after nebulization in Balb/c mice. Results: Formation of FA-NLCs which exhibited a controlled release profile, was confirmed by scanning electron microscope and differential scanning calorimetry. FA-NLCs exhibited toxic effects on A549 cells for longer time periods as compared to FA solution. Following the aerosolization, suitable aerodynamic properties were obtained and FA-NLCs formulation provided significantly increased residence time and slower lung clearance for FA. Further confocal microscopy visualization confirmed the lung deposition of nanoparticles. Encapsulation of FA into the NLCs resulted in the improved pharmacokinetic parameters in plasma or lung tissue samples. Conclusion: Application of the aerosolized FA-NLCs formulation which improves the pulmonary bioavailability of FA might result in the increased efficiency and reduced dosing frequency of this phytochemical. In this respect, development of inhalable nano-based drug delivery systems appears as a promising therapeutic approach against the lung disorders.


Abbas Alimoradian, Hadi Ansarihadipour, Saeed Changizi-Ashtiyani, Ali Chehrei, Reza Talebi, Sadaf Davudian, Soheila Rostami,
Volume 22, Issue 1 (Winter 2018)
Abstract

Introduction: The stress-oxidative is involved in doxorubicin (DOX)-induced cardiotoxicity. Due to the potential and previous reported for antioxidant properties of atorvastatin, omega-3, vitamin E and vitamin C, their efficacy to prevention of DOX-induced cardiotoxicity was investigated in this study. Methods: Fifty-six male rats were divided into 8 groups which received omega-3, atorvastatin, vitamin E, vitamin C, normal saline and dimethyl sulfoxide (DMSO) via gavage for 14 days then a single dose of DOX (20 mg/kg) was injected intraperitoneally except two last groups that received only normal saline or DMSO. The level of oxidative stress parameters like ferric reducing ability of plasma (FRAP) before and after DOX injection and malondialdehyde (MDA) of heart were estimated. Also the histopathologic assessments were done on heart sample at the end of experimental period. Results: The results showed that compared to other agents, omega-3 could emerge as the most protection against DOX. Its pretreatment led to one of the most FRAP changing percent meanwhile less MDA value and cardio pathologic indexes almost close to control groups compared to that of other agents (P<0.01). Conclusion: Omega-3 may have a promising protective effect against DOX-induced cardio toxicity.



Page 1 from 1