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Showing 3 results for Namvar

Maryam Zeraati, Javad Mirnajafi-Zadeh, Mohammad Javan, Saeed Semnanian, Simin Namvar,
Volume 14, Issue 2 (Summer 2010)

Introduction: Considering high prevalence of epileptic disease and considering that 40 percent of epileptic patients are resistant to drug therapy, it needs more researches to find new therapeutic ways. LFS is among the new methods for epilepsy treatments. One possible mechanism involved in the anticonvulsant effect of LFS is increased adenosine. Therefore, in this study the role of adenosine production from ATP by ectonucleotidase enzyme pathway in exerting the anticonvulsant effects of LFS were evaluated. Methods: Animals were kindled by electrical stimulation of perforant path in a rapid kindling manner (12 stimulation per day). One group of animals received LFS after kindling stimulation. In one another group, AOPCP a blocker of ectonucleotidase inhibitor was micro injected (50 micro molar) intra cerebro ventricular each day before LFS stimulation. Some group of animals were also received AOPCP (50 and 100 micro molar) but were not applied to LFS. Seizure behavior and electrophysiological parameters (including ADD and field potential) were recorded. Results: Like previous investigations, application of LFS, decreased all seizure parameters significantly. Microinjection of AOPCP had no significant effect on anticonvulsant actions of LFS. However microinjection of AOPCP at doses of 100 micro molar in animals that received just kindling stimulations, increased the seizure parameters significantly. Conclusion: The results show that adenosine production via ectonucleotidase enzyme pathway may has no role in anticonvulsant effects of LFS however endogenous adenosine produced through this pathway has an important role in kindling development.
Simin Namvar, Javad Mirnajafi-Zadeh, Mohammad Javan, Maryam Zeraati,
Volume 14, Issue 3 (Fall 2010)

Introduction: Application of low-frequency stimulation (LFS) is a new method for treatment of drug resistant epileptic patients. Previous studies demonstrated that activation of receptors coupled to Gi proteins is one of the mechanisms of the anticonvulsant effect of LFS. Thus, in this study, alterations in the expression of RGS4 and RGS10 proteins, as negative regulators of Gi proteins, were investigated. Methods: Animals were kindled by perforant path stimulation in a rapid kindling manner (12 stimulation per day, 1 ms pulse duration at 50 Hz). LFS (8 stimulation per day, 0.1 ms pulse duration at 1 Hz, 200 pulses) was applied to the perforant path 5 minute after the termination of kindling stimulations. After electrophysiological recordings for 6 days, the dentate gyrus of the animals was removed and RGS4 and RGS10 protein expression was studied by western blotting technique. Results: Application of LFS significantly retarded kindling acquisition and increased the number of stimulations to achieve different stages of seizure. LFS also significantly reduced after discharge duration. In addition, application of LFS after kindling stimulation reduced the expression of RGS4 and RGS10 proteins. Conclusion: Results of the present study showed that LFS has anticonvulsant effects on the perforant path kindling. Application of LFS following kindling stimulation reduced the expression of RGS4 and RGS10 proteins. Reduction of the expression of these proteins results in the longer activation of signaling pathways of Gi proteins, which may be responsible for LFS anticonvulsant effects.
Nahid Sarahian, Hedayat Sahraei, Homeira Zardooz, Hengameh Ali-Beik, Bahareh Sadeghi, Taherehsadat Javadifar, Gholamreza Herfehdoost, Badri Zarrin Ehteram, Zahra Ghanbari, Fatemehsadat Hoseini-Namvar,
Volume 18, Issue 4 (Winter 2015)

Introduction: In this study, the effect of memantine administration into the nucleus accumbens on the metabolic changes induced by acute stress in female mice was evaluated. Methods: Intra-accumbens unilateral or bilateral canulation was performed. One week after recovery, a group of animals were given memantine (1, 0.5, and 0.1 μg/mouse) five min before stress induction intra-accumbally, and the other group received it (1, 0.5 and 0.1 mg/kg) 30 min before stress intraperitoneally. Food and water intake, weight of fecal material, and the delay time before eating were measured as metabolic parameters after stress induction. Results: Acute stress reduced water and food intake, fecal matter, and the delay time before eating. Intraperitoneal memantine injections augmented the stress effect on water intake, but inhibited its effect on food intake at dose of 0.1 mg/kg and had no impact on defecation. The drug induced anorexia especially at dose of 1 mg/kg. On the other hand, intra-accumbens memantine injections reduced water intake when the drug was injected in the left side. Moreover, memantine injections inhibited or enhanced the effects of stress on water intake, food intake and defecation in a doseand location-dependent manner, and also increased the delay time before eating. Conclusion: Memantine inhibits or enhances the effects of acute stress dose-dependently. In addition, it seems that there is asymmetry in nucleus accumbens response.

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