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Showing 8 results for Kesmati

Zahra Abbasi Zadeh, Mahnaz Kesmati, Hadi Fathi Moghaddam,
Volume 10, Issue 3 (Fall 2006)

Introduction: In this study the effect of Matricaria recutita extract on acute pain in the presence and absence of flumazenil (antagonist of benzodiazepine) and lesioned paragigantocellularis (PGi) nucleus was investigated. Method: Wistar male rats (250±20 g) were used. Animals were grouped randomly into intact, sham and bilateral lesion of PGi nucleus. Intact and lesioned animals, each divided into 4 subgroups which received saline, M. recutita hydro-alcoholic extract (25 mg/kg), flumazenil (1 mg/kg) and flumazenil + M. recutita. Hot plate test were used for pain thereshold evaluation. Results: Results showed that: In intact animals M. recutita induced analgesic effect but flumazenil had significant hyperalgesic effect. In these animals flumazenil prevented the analgesic effects of M. recutita. Lesion of PGi induced significant hyperalgesia. M. recutita in absence of PGi nucleus showed significant analgesic effect and flumazenil increased pain sensation and caused significant hyperalgesia. The analgesic effect of M. recutita attenuated by flumazenil in absence of PGi nucleus. Conclusion: It seems that PGi nucleus involves in pain modulation but analgesic effect of M. recutita that may be via the benzodiazepine-like activity of some its components does not interact with PGi nucleus.
Khadije Gholami, Mahnaz Kesmati, Reza Kazeminejhad, Faride Zangene, Abdoalrahman Rasekh,
Volume 11, Issue 1 (Spring 2007)

Introduction: Some studies indicate changes in the level of thyroid hormones in addicted people. Also, there are some reports concerning the modulation of hypothalamus-hypophysis-thyroid axis in the context of morphine addiction. In the present study, the effects of thyroid gland activation via the acute and chronic administration of levothyroxine on morphine withdrawal syndrome were investigated. Methods: Frothy two adult male mice were divided into 6 groups. Animals received three daily injections of morphine (20, 40, 80 mg/kg) alone or in combination with l-thyroxin (0.5 mg/kg, i.p: single dose, chronic for 4 days and chronic for 16 days). Morphine withdrawal syndrome was induced using naloxone. Withdrawal signs such as jumping, rearing, climbing and weight loss were recorded for 30 minutes. Results: The results showed that acute levothyroxine administration increased the morphine withdrawal signs. Four days administration of levothyroxine reduced the number of jumping and climbing and inhibited weight loss. Administration of levothyroxine for 16 days reduced only the number of rearing. Conclusions: It seems that levothyroxine via alteration of thyroid hormones level can affect morphine withdrawal signs and this effect of levothyroxine can be attenuated with its repetitive administration.
Sepideh Khaksar, Mahnaz Kesmati, Anahita Rezaie, Abdolrahman Rasekh,
Volume 14, Issue 3 (Fall 2010)

Introduction: Impaired wound healing in diabetic patients is a major clinical problem, which is associated with significant morbidity and mortality. Estrogen has positive effects on neoangiogenesis, reepithelialization and cell proliferation. In this research, effect of estrogen on wound healing in diabetic male rats was investigated. Methods: This study was performed on male Wistar rats (body weight 200±20 g), which were divided into 2 groups of normal and diabetic rats. Each group was divided into 3 subgroups of control, sham and test. A circular full-thickness wound with a diameter of 1.5 cm was created on the back of streptozotocin(stz)-induced diabetic as well as nondiabetic rats. Estradiol benzoate (10 μg/sc) was daily administered to test subgroups for 28 days, while the sham subgroups received injections of placebo. The control subgroup did not receive anything. Size measurement and pathological evaluation of the wound was performed on days 3, 5, 7, 14, 21, 28. Results: In the macroscopic study, there was a delay in the wound healing of diabetic group in comparison with normal group. From day 7, wound healing had considerable change in estradiol subgroups in both normal and diabetic rats (p<0.05). In the microscopic study, coating tissue reorganization, granulation tissue and neoangiogenesis formation were surveyed as semi-quantitative parameters. In all cases, estradiol receiving subgroups showed impressive improvement compared to the sham subgroup. Conclusion: This research finds that estrogen can improve the impaired wound healing of diabetic rats and this effect is related with the rate of wound healing and wound structure.
Sara Pourabbas, Mahnaz Kesmati, Abdolrahman Rasekh,
Volume 15, Issue 1 (Spring 2011)

Introduction: Fennel is rich in phytoestrogens and is used for estrogen deficiency disorders. Estrogens affect anxiety through neurochemical systems such as GABA-A receptors. In this study the effects of fennel on GABA-A and estrogen receptors in anxiety were investigated. Methods: Adult female Wistar rats weighing (180±20 g) were divided into 8 groups. Groups received saline, fennel (200, 500, 750 mg/kg), tamoxifen (15 mg/kg) + fennel (500 mg/kg), picrotoxin (1 mg/kg) + fennel (500 mg/kg). A control group was also used. Elevated plus maze was used for evaluation of anxiety by measuring the time spent in the open arm. All drugs were administered intraperitoneally. Results: The results showed that fennel only at the dose of 500 mg/kg had significant anxiolytic effects and increased the time spent in open arms (P<0.01). Picrotoxin (GABA-A antagonist) significantly prevented anxiolytic effect of 500 mg/kg of fennel (P<0.001). Tamoxifen, an estrogen receptor antagonist, also abolished the anxiolytic effect of fennel (P<0.001). Conclusion: Fennel reduced anxiety in rats and picrotoxin, a non-competitive antagonist of GABA-A receptors, as well as tamoxifen, an antagonist/agonist of estrogen receptors, reduced this anxiolytic effect. Thus fennel as a herbal drug seems to have an anxiolytic effect and it probably acts through GABA-A and estrogen receptors.
Mahnaz Kesmati, Mozhgan Torabi, Hamid Malek Shahi Nia, Hossein Teymuri Zamaneh,
Volume 16, Issue 4 (Winter 2013)

Introduction: The use of zinc supplements can decrease somatic and visceral pains. Exercise also increases the pain tolerance. In recent years, production and use of nano metal particles such as nano ZnO for pharmaceutical, hygienic and industrial purposes is rapidly increasing, while studies about its effects on human health are limited. The aim of the present study was to compare the effect of chronic administration of nano and conventional ZnO on nociception in the presence and absence of anti nociception mechanisms induced by aerobic exercise. Methods: In this investigation, adult male Wistar rats (185±10 gr) were used in the following groups: Control (receiving saline, without exercise), exercise, conventional ZnO and/or nano ZnO daily (1 mg/kg i.p., 5 days a week) for 6 weeks with and without aerobic exercise. Exercise groups did daily protocol of exercise for 30 minutes after drug injection. In the end, analgesia time was evaluated by hot plate test. Results: Exercise, ZnO and nano ZnO significantly reduced pain (P<0.01, P<0.05, P<0.01). Nano ZnO showed a better analgesic effect in comparison to conventional ZnO, but this difference was not significant. Similarly, no significant difference was found between the group that had only exercise and groups treated with ZnO and/or nano ZnO. Conclusion: Antinociception mechanism induced by exercise is not potentiated with nano and conventional ZnO.
Marzieh Khorshidi, Mahnaz Kesmati, Lotfollah Khajeh Pour, Hossein Najaf Zadeh Varzi,
Volume 17, Issue 2 (Summer 2013)

Introduction: With the increasing development of nanotechnology, nanomaterials are used instead of conventional compounds. One of these nanomaterials that have many applications in the biomedical field, is iron oxide (Fe2O3) nanoparticles and there is not much research on its effects on the physiological features. So in this research, effect of iron oxide nanoparticles on short and long-term passive avoidance memory and levels of serotonin and dopamine in hippocampus was evaluated in comparison to the bulk type. Methods: In this study, 80 male adult Wistar rats (220±30grams) were used and divided into 10 groups, and the study was performed in two parts in order to measure the behavioral changes and neurotransmitters levels. In the first part, animals received intraperitoneal injections of iron oxide nanoparticles and bulk at different doses (0.2, 1, 5 mg/kg( before training. Then, 90 minutes and 24 hours after training their memory was tested by a step–through instrument. In the second part of the study, right and left hippocampi of every group were extracted after receiving the effective dose (5 mg/kg) of iron oxide nanoparticles and bulk, and neurotransmitters levels were measured by an ELISA kit. Results: Iron oxide nanoparticles caused a disruption in passive avoidance memory in a dose-dependent manner, while bulk iron oxide showed a non significant partial effect. Also both type of iron oxide reduced dopamine levels and increased serotonin levels significantly or relatively in both hippocampi. Conclusion: It seems that iron oxide nanoparticles induce a disruption of memory, which is in part related to the alterations of neurotransmitters levels in hypocampus and the other is associated to its physicochemical properties.
Zahra Abbasizade, Mahnaz Kesmati, Hamid Galehdari, Anahita Rezai, Seyed Mansor Seyednezhad, Mozhgan Torabi,
Volume 18, Issue 1 (Spring 2014)

Introduction: Several factors such as diseases, medications and humoral agents can delay or speed up wound healing process. Because of the limited information about the hormonal changes during wound healing, in this study the relationship between serum levels of growth hormone, insulin and cortisol and wound healing in normal and diabetic rats was evaluated. Methods: Male Wistar rats )weighing 250 ± 20 g( were divided into three groups: control, normal (non-diabetic) and diabetic (induced by streptozotocin). The wound size made on the back of normal and diabetic rats was measured at days 0, 7, 14 and 21, and the serum levels of growth hormone, insulin and cortisol were measured. Results: The speed of wound healing in normal rats was higher than diabetic rats. Serum insulin concentrations were less in the diabetic rats in comparison with the normal and control groups (p<0.001, and showed correlation with wound healing process in diabetic rats (p<0.01). Serum cortisol concentrations decreased in normal and diabetic groups during wound healing (p<0.001) but did not show significant correlation with this process. Serum growth hormone levels did not significantly change in any of the groups, and did not show significant correlation with wound healing process, too. Conclusion: Reduction of serum insulin level is probably responsible for delayed wound healing in diabetes, and intrinsic mechanisms facilitate wound healing in normal and diabetic conditions by reducing the release of cortisol.
Mahnaz Kesmati, Maryam Konani, Mozhhgan Torabi, Lotfollah Khajehpour,
Volume 20, Issue 3 (September 2016)

Introduction: Our previous study has showed that chronic administration of magnesium oxide nanoparticles (MgO NP) can reduce anxiety in adult male rat. In this study the effects of MgO NP on anxiety induced by morphine withdrawal were investigated in adult male mice. Methods: Adult male NMRI mice (weighing 27 ± 3 g) divided into groups: control, receiving intraperitoneal (i.p.) injection of MgO NP (1, 2.5, 5 mg/kg), morphine withdrawal groups that receiving saline or MgO NP (2.5, 5 &10 mg/kg) as acute (a single injection at the test day) and chronic (co-injected with morphine for 4 days). To develop morphine dependency, increasing doses of morphine (20, 40, 80 mg/kg( injected subcutaneously for 4 days. Mice received a final morphine injection (40 mg/kg) 3 hours prior to naloxone (5 mg/kg (i.p.) on the day of testing (day 4). In addicted groups, after naloxone injection, morphine withdrawal signs were evaluated. In all groups, anxiety like behavior was assessed by the elevated plus maze apparatus. Results: MgO NP (2.5 & 5 mg/kg) reduced anxiety like behavior (P<0.05). Acute and chronic MgO NP injections (5&10 mg/kg) could significantly improve/alleviate anxiety like behavior (p<0.05 & p<0.01 respectively) and reduce locomotor activity (p<0.05, acute; p<0.05, & p<0.01, chronic), rearing, climbing and weight loss in morphine withdrawn mice. Conclusion: Due to the positive effect of MgO NP on anxiety like behavior and morphine withdrawal signs and symptoms, this nanoparticle can be a potential candidate for reducing the side effects of chronic usage of morphine and morphine withdrawal.

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