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Showing 10 results for Fereidoni

Masoud Fereidoni, Mohammad Javan, Saeed Semnanian, Abolhasan Ahmadiani,
Volume 10, Issue 4 (Winter 2007)

Introduction: Different mechanisms are involved in stress induced analgesia (SIA) and hyperalgesia (SIH). Repeated stress induces development of tolerance to SIA. The role of HPA axis and Gs signaling pathway in these effects are investigated in the current study. Methods: Forced swim stress (5 min/day) in water (20±1 ºC) was employed to adult male Wistar rats (200-250 g). The nociceptive threshold was assessed using tail flick test. Adrenalectomized (ADX) rats were also subjected to stress tests. Oseltamivir was used to block Gs signaling pathway. Results: Stress produced analgesia for 1 h (p<0.001) and hyperalgesia during 3-24 h after its induction (p<0.05). Repeated administration of the stress caused tolerance development to SIA and increased SIH recorded at 24 h after each session (p<0.001). Oseltamivir couldn’t reverse the SIH. Dexamethasone produced hyperalgesia from 30 min (p<0.001) to 24 h after its administration (p<0.01). Repeated injection of dexamethasone increased the hyperalgesia recorded at 24 h after treatment (p<0.001). In ADX animals SIA continued for 24 h (p<0.01). Adrenalectomy attenuated the chronic stress-induced SIA tolerance and eliminated SIH. Conclusion: SIH is suggested to be related to adrenal activity which also has a role in SIA tolerance. Upper parts of HPA axis seems to be responsible for SIA. Oseltamivir could not reverse the SIH. Therefore, the Gs signaling pathway activation by opioid system may not be responsible for SIH.
Vaezi Gholamhasan, Shahrbanoo Oryan, Masoud Fereidoni, Leila Etemadi, Fereshte Manafi,
Volume 11, Issue 2 (Summer 2007)

Based on the extensive application of Peganum harmala (P.h) seeds in the Asian traditional medicine, we tried to investigate its possible anxiety effect. Method: The effect of P.h. extract inhalation was evaluated in adult male rats using elevated plus-maze apparatus. The humidity of prepared ethanol extract was 37%. Animals in different groups (n=6) received 2, 4, 6, 12 or 18 gr/ml doses of the extract using Nebulizer. harmaline drug (0.13 gr/ml) was used as positive control drug. Results: Compared with saline treated group, harmaline as the positive control significantly caused fear in rats as it was shown by increased time spent in closed arm of plus-maze (p< 0.05). Also, ethanol extract of P.h was able to show anxiety effect at doses 6, 12 and 18 mg/ml (p <0.05). Conclusion: Our data showed effective anxiety effect of ethanol extract of Peganum harmala. Its effect should be considered in the context of its extensive usage in the men daily life. More studies are required to elucidate its mechanism and site of action.
Vaezi Gholamhassan, Masoud Fereidoni, Leila Etemadi, Maryam Sabzali,
Volume 11, Issue 4 (Winter 2008)

Traditionally, Peganum harmala seeds (P.h) have been extensively used in the Asia region. We have previously reported the increase of fear behavior by systemic administration of P.h extract. Here, we evaluated the effect of central administration of the extract on the fear behavior. Method: Methanolic extract of the plant's seeds (37% humidity) was prepared for the investigation. Elevated plusmaze apparatus was used for evaluating the fear behavior. Adult male rats were categorized in 7 main groups (n=6). 1) Sham control (saline 1 ul/rat, i.c.v) 2) Harmaline treated group (50 ug/rat, i.c.v). 3) Extract treated groups (10, 20, 25, 50, 100 ug/rat i.c.v respectively). Results: All the doses of the P.h Methanolic extract as like as harmaline caused fear behavior (p<0.05). There was no significant difference between the effects of harmaline and the doses of the plant extract. Discusion: Overall, it is possible that the main alkaloid of the P.h (harmaline) is responsible for the increasing of fear behavior. The effect seems to be done trough the central nervous system neurochemical mechanisms.
Masoud Fereidoni, Leila Etemadi,
Volume 12, Issue 2 (Summer 2008)

Introduction: Feverfew (Tanacetum parthenium) (T.p.) is widely used in folk medicine to treat many diseases. We reported the analgesic effect of T.p. flower and leaf previously. Present study is designed to find the mechanism underlying the anti-nociceptive effect of the aqueous extract of T.p. flower. Method: Based on our previous study, the dose 50 mg/kg i.p. of the T.p. aqueous extract had a potent analgesic effect on mice (NMRI) (20 ± 2 g) in formalin test which is used in the present study also. Here, we study the roles of opioidergic, sertoninergic and α - adrenergic systems on the anti-nociceptive effect of the extract. Animals had pretreated with drugs, 15 min before the extract treatments, including opioid antagonist naloxane (5mg/kg, i.p.), sertoninergic antagonist cyproheptadine (4 mg/kg, i.p.) and α-adrenergic antagonist phentolamine (20 mg/kg, i.p.) separately (each group with n≥6). Saline and extract used as controls. Results: In contrast to extract analgesic effect, pretreatment with naloxan increased the pain sensation in the neurogenic phase of formalin test (p<0.001). Pretreatment with cyproheptadine increased the sensation of pain in both early and late phases (p<0.05). Inhibition of α -adrenergic system was not be able to attenuate the anti-nociceptive effect of the extract. Discussion: The involvement of sertoninergic system in anti-nociceptive effect of the T.p. extract is proposed by the results. Also the involvement of opioidergic system has to be mentioned in this effect.
Azam Shafaie, Masoud Fereidoni, Ali Moghimi, Morteza Behnamrasooli,
Volume 12, Issue 4 (Winter 2009)

Introduction: In the Kindling-induced seizure model, low and repeated electrical or chemical stimulations, can elevate the neural network excitability and induce epileptiform seizures. Opioid receptors are widely distributed in different areas of the brain. On the other hand, morphine has paradoxical effects and induces elevation or alleviation of the pain sensation and excitability, at different doses. The present study is designed to investigate the effect of ultra low dose morphine on seizures induced by pentylentetrazol (PTZ). Methods: PTZ (32 mg/kg i.p.) was administered for 12 constitutive days to kindle the male Wistar rats (200-250 g). Animals were treated by saline or morphine (0.1 μg/kg, 1 μ g/kg, 10 μ g/kg and 10 mg/kg), 30 min before PTZ administration (n = 7-9) and seizure severity was recorded during 30 min after PTZ administrations. Results: Morphine at the dose of 10 mg/kg was able to elevate the seizure intensity and accelerate the kindling process (p<0.001), but at the dose of 10 μg/kg, attenuated the seizure intensity and kindling development (p<0.05). Conclusion: The reason for this paradoxical effect of morphine on PTZ-induced seizure could be that morphine, at ultra low doses, can elicit the stimulatory signaling pathway of Gs protein, rather than the inhibitory Gi pathway. It seems that ultra low does of morphine by inducing the activity of Gs signaling can lead to the attenuation of PTZinduced seizures, while activation of Gi signaling using ordinary doses of morphine can cause potentiation of PTZinduced seizures.
Azam Sadegh, Masoud Fereidoni, Ali Moghimi,
Volume 14, Issue 2 (Summer 2010)

‎ Introduction: Controversial results have been reported about the effect of morphine and stress on learning and spatial memory in rodents. There are very few studies about the effects of ultra low doses of morphine on memory. In this study, effects of acute administration of low and usual doses of morphine on memory formation and retention in the presence and absence of repeated stress were investigated. Methods: adult male Wistar rats (200-250g) were divided into 3 groups A) Rats were trained for 4 constitutive days and then intraperitoneally received different doses of morphine (1μg/kg, 10μg/kg, 100μg/kg, 1mg/kg and 10mg/kg) 30 minutes before retention test on the 5th day. B) Animals experienced forced swimming stress 30 minutes before each training session for 4 constitutive days and memory retention was evaluated on the 5th and 12th days. C) Rats were treated like animals in group B and then like group A. In all groups, retention tests were done without any excessive treatment on the 12th day. Escape latency and mean path length from the starting point to the platform on training days were considered as learning parameters, while time spent in the target quadrant on the 5th and 12th days was regarded as retention parameter. Results: Memory retention was decreased with 1 μg/kg and 10 mg/kg doses of morphine on the 5th day (P<0.001). Repeated stress led to decreased learning (P<0.001) and retention on the 5th and 12th days (P<0.05). In animals treated with both repeated stress and acute morphine (except for the dose of 1 mg/kg) retention decreased on the 5th day (p<0.001), while retention diminished for all groups on the 12th day. Conclusion: Morphine at usual dose of 10 mg/kg may cause memory retention impairment, by its inhibitory action on the opioidergic system. Surprisingly, morphine at ultra low dose (1 μg/kg) has the same effect and the excitatory action of opioidergic system may be responsible for this effect, however it needs further studies. Repeated stress in combination with morphine even at ineffective dosage could cause memory impairment in the Morris water maze, so the presence of both factors, can probably cause additive impairment of memory
Malihe Ekandari, Masoud Fereidoni, Ali Moghimi,
Volume 14, Issue 4 (Winter 2011)

Introduction: Pain research using animal models is related to ethical concerns, so invertebrates and insects have been recommended by researchers. In the present study, the nociceptive and antinociceptive effects of capsaicin, aspirin, morphine and chili extract were examined using fruit fly (Drosophila melanogaster) as an alternative for rodent pain model. Methods: Stage 3 of larvae and adult state of Drosophila were used. Threshold and maximum reactions were recorded in thermal nociception (using Hot plate) and chemical nociception (Writhing test) at various concentration of acetic acid, capsaicin and chili hydro alcoholic extract. Rolling movement responses were recorded in separate groups (n=7). In adult Drosophila, latency for heat tolerance on hot plate was recorded (n=10), and the effect of morphine and aspirin at different concentrations on pain were examined. Results: Increasing the temperature, acetic acid, capsaicin and chili extract concentration, attenuated the rolling movement in larvae. In adult drosophila, increasing the temperature, diminished tolerance latency on hot plate. In this animal model, increasing the morphine and aspirin concentration diminished responses to pain stimulus. Conclusion: According to our results, it can be suggested that Drosophila melanogaster can be used as a model for investigation on pain physiology and antinoiciception. Painless channel (closest vertebrate homolog of TRAP1 channel) maybe required for thermal and chemical nociception in drosophila. Similar to mammals, treatment with morphine and aspirin may exert these effects through Gi and cox2 respectively.
Shirin Hosseini, Farzaneh Sabouni, Masoud Fereidoni, Ali Moghimi,
Volume 16, Issue 2 (Summer 2012)

Introduction: Astrocytes have an important role in many neurodegenerative diseases. Active astrocytes release inflammatory factors such as NO and ILs. Shikonin, a naphthoquinone pigment of Lithospermum erythrorhizon roots has anti-inflammatory and antitumoral effects. The present study aims to investigate the anti-inflammatory and toxic effects of Shikonin on cultured astrocytes. Methods:Two-day-old rat infants' brains were homogenized after removal of the meninges, and cultured in DMEMF12+10% FBS medium. Ten days later, astrocytes were harvested and re-cultivated for more purifications to 95%, using immunocytochemistry method, and then treated by different concentrations of shikonin (0.1, 0.5, 1, 2.5, 5, 10, 20, 30, 50 and 100μM) for one hour, which was followed by LPS exposure. Viability was measured by MTT assay and NO concentrations were assessed by Griess method, to reveal the inflammation process, after 24 and 48 hours. Results:Shikonin concentrations of 1, 2.5, 5, 10, 20 and 30μM had no anti-inflammatory and cell death effects but at 0.1 and 0.5μM showed a significant anti-inflammatory effect and ability to reduce NO production by astrocytes (p<0.001) and at 100 and 50μM showed a significant cell death effect and NO production reduction (p<0.001). Conclusion:Reduction of NO production might be due to inhibition of release and expression of inflammatory and cell signaling factors such as ILs and iNOS under the shikonin anti-inflammatory effects at concentration around 0.1 and 0.5μM. However, inhibition of NO production by shikonin at 50 and 100μM is probably due to cell death induction.
Zohreh Abbasi, Masoud Fereidoni, Morteza Behnam-Rassouli,
Volume 17, Issue 2 (Summer 2013)

Introduction: Uncoupling protein 2 (UCP2) in the inner mitochondrial membrane changes the activity of KATP channels and the cell excitability, probably by decreasing the ATP production. Given the expression of UCP2 in primary afferent neurons, and the importance of these channels in morphine-induced analgesia, genipin, an UCP2 inhibitor, may affect these processes, when administrated intrathecally. Methods: Tail flick assay and formalin test were used to study thermal and chemical pain, respectively, in adult male Wistar rats. Catheterization of the spinal subarachnoid space was used to localize the effects of genipin. Results: Genipin increased pain sensation in tail flick assay and in the first phase of the formalin test, but decreased pain in the second phase of the formalin test (P<0.001 in all groups). Genipin also antagonized analgesic effect of morphine (P<0.01 in tail flick and P<0.001 in the first phase of the formalin test) and did potentiate it in the second phase of the formalin test (P<0.001) but had no effect on hyperalgesic effects of ultra-low dose of morphine. Conclusion: Probably, genipin increases ATP/ADP ratio, thereby inhibits KATP channels in primary pain afferents and lowers pain threshold and decreases analgesic effects of morphine, at least in part, by inhibition of UCP2. Apparently, the anti-inflammatory effect of genipin causes analgesia in the formalin test. Genipin had no effect on hyperalgesic effects of ultra-low dose of morphine, maybe due to the common signaling mechanisms such as KATP channels.
Narjes Lotfi Ghadikolai, Masoud Fereidoni, Ali Moghimi,
Volume 17, Issue 4 (Winter 2014)

Introduction: Ethanol can induce a wide spectrum of neurophysiological effects via interaction with multiple neurotransmitter systems and disruption of the balances between inhibitory and excitatory neurotransmitters. Prefrontal cortex is involved in cognitive process including working memory and is sensitive to ethanol. Present study investigates the effects of intraperitoneal (i.p.) administration of multiple doses of ethanol and intra-prefrontal cortex (i.c.) administration of ethanol on spatial working memory performance. Methods: Adults male wistar rats (200-250g) were used. Rats in various groups received saline (i.p.), saline (i.c.), ethanol (10%, 20% and 30%, i.p.) and ethanol (30%, i.c.). Surgery for intra-prefrontal cortex cannulation was performed for i.c. administrations. The spatial working memory was assessed 15 and 30 minutes after i.p and 5 minutes after i.c. injections at first, second, and third day using the 8-shape maze apparatus. Results: Ethanol (10%, i.p.) had no significant effects, but 15 and 30 min after administrations of ethanol (20%, i.p.) (P<0.05), and 15 min after administration of ethanol (30%, i.p.) (P<0.001), spatial working memory performance decreased significantly. Only i.c. administration of ethanol 30% at the first day, diminished working memory performance (P<0.001). Conclusion: Because working memory was impaired in both intraperitoneal and intra-prefrontal cortex administration, therefore probably a part of spatial working memory deficits is related to effects of ethanol on prefrontal cortex and disruption of neuronal circuits involved in working memory in this region.

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