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The effect of saffron aqueous extract on oxidative stress parameters and important biochemical enzymes in the testis of streptozotocin-induced diabetic rats. Physiol Pharmacol. 2017;
URL: http://phypha.ir/ppj/article-1-1294-en.html

Abstract:   (50 Views)

Introduction: Oxidative stress is responsible for complication in diabetes including impotence and infertility. Saffron had antidiabetic and antioxidant properties that improve the functions of various organs affected by hyperglycemia. The aim of the present study was to investigate the beneficial effects of administration of saffron aqueous extract in streptozotocin (STZ)-induced diabetic rats.

Methods: The fasted rats were injected by a single intraperitoneal injection of a freshly prepared solution of STZ (65mg/kg body weight) in 0.1M cold citrate buffer (pH =4.5). 72 hours after STZ administration, the animals with fasting blood glucose concentrations of over 250mg/dl were considered to be diabetic and were used in the experimental groups; normal control (1), diabetic control (2), saffron control (3) and saffron treated (4). The treatment was started on the 7th day after STZ injection with i.p injection of Saffron (200mg/kg body weight), five doses and weekly to groups (3 and 4). At the end of the experimental period, biochemical parameters were determined in testis tissues.

Results: Administration of saffron to diabetic rats significantly decreased blood glucose and prevented weight loss in saffron treated rats (group 4). Results showed saffron administration decreased elevated biochemical enzymes levels in testes of diabetic rats. Also, saffron significantly increased CAT and GPx activities in testes of diabetic rats. MDA levels had no significant changes in all experimental groups.

Conclusion: The results demonstrate that saffron administration controlled blood glucose and also, it improved antioxidant enzymes function against oxidative stress via its antioxidant properties.

     
Types of Manuscript: Original Research | Subject: Endocrinology