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Talebianpoor M S, Owji S M, Goharinia M, Mirkhani H. Effect of Tempol, a synthetic antioxidant, on renal complications of L-NAME induced preeclampsia in rat. Physiol Pharmacol. 2017;
URL: http://phypha.ir/ppj/article-1-1254-en.html

Abstract:   (232 Views)

Introduction: It has been suggested that oxidative stress has a crucial role in the pathophysiology of preeclampsia. In the present study, the effect of tempol, a synthetic antioxidant, on kidney injuries and oxidative stress was investigated in an experimental model of preeclampsia in rats.

Methods: Preeclampsia was induced by oral administration of L-NAME to the rats on the day 10 of pregnancy. Animals were randomly divided into six groups (10-15 rats in each group); (1) Normal pregnant (II) Preeclamptic (III, IV, V) Preeclamptic + Tempol 20, 60 and 180 mg/kg/day, respectively, (VI) Preeclamptic + Hydralazine 10 mg/kg/day. Urine levels of sodium, potassium, creatinine, lactate dehydrogenase and 24 h protein, blood levels of creatinine and urea, in addition to malondialdehyde concentration in blood and kidney, as well as histological glomeruli changes were assessed.

Results: L-NAME administration caused proteinuria and glomerular pathological changes. Tempol (20 and 60 mg/kg/day) significantly reduced plasma and renal (P<0.001) malondialdehyde levels and proteinuria (the biggest calculated P-value was less than 0.05) in preeclamptic rats. Tempol at the dose of 20 mg/kg/day improved the histological changes in preeclamptic animals, but the dose of 60 mg/kg/day restored histological findings. L-NAME did not change the other measured parameters. Hydralazine and highest dose of Tempol (180 mg/kg/day) failed to affect biochemical and histological changes in experimental preeclampsia.

Conclusion: Renal complications of experimental preeclampsia such as proteinuria and glomerular injuries can be prevented by Tempol. The desired effects of Tempol depend on its dose.

Type of Study: Original Research | Subject: Pharmacology