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Abstract:   (266 Views)
Introduction: The use of standard rodent model, allows for the understanding of neuronal injury physiopathology, and helping development of therapeutic strategies. According to contusion type of spinal cord injury (SCI) and mild traumatic brain (TBI) injuries that are commonly occurred in the human, we designed a novel impactor device with ability to induce different degrees according to kilodyne from very mild to very severe of SCI and TBI models in rats. Methods: For standardization and determining of optimal performance of the device to induce varying injuries, 47 adult male Wistar rats were used, and 8 different forces were applied in spinal cord and brain tissues. Results: The Hematoxylin and Eosin (H&E), and 2, 3, 5-triphenyltetrazolium chloride (TTC) results demonstrated that by increasing the level of forces, histological changes in the spinal cord and brain were significantly enhanced. Different injuries had significant effect on the Basso, Beattie, and Brenham (BBB) and elevated body swing test (EBST) outcomes, and there were significant differences between groups in comparison with control group. Conclusion: Our results show that the novel device could be valid to produce precise SCI and TBI models, goal to replicate SCI and TBI in humans as much as possible. However, it might be considered that aspects of SCI and TBI models are complicate and more examination is necessary.
     
Types of Manuscript: Original Research | Subject: Nervous system (others)