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Abstract:   (204 Views)
Introduction: Ginger has shown anti-nociceptive effects. Here we investigated the possible involvement of GABAA receptors in anti-nociceptive effect of ginger by using muscimol (GABAA agonist) and picrotoxin (GABAA antagonist) in rats that received ginger. The pain sensitivity was evaluated by formalin test. Methods: Thirty-five male Sprague-Dawley rats were randomly divided into 7 groups (n=5): Sham 1 (received distilled water. P.O.); Sham 2 (received water + 0.75 µl atrtificial cerebrospinal fluid (ACSF) by intracerebroventricular (ICV) injection; experimental 1 (received ginger at 50 mg/kg/day, P.O.); Experimental 2 and 3 (received ginger + 0.75 µl of 250 or 500 ng/rat muscimol by ICV injection); Experimental 4 and 5 (received ginger + 0.75 µl of 250 and 500 ng/rat picrotoxin by ICV injection). On day 16 and 30 min after ICV injections, formalin test was performed on all rats. Results: Ginger significantly reduced pain sensitivity in both phases of formalin test in comparison to sham 1 and 2. In early and late phase, both doses of muscimol reduced pain sensitivity as compared to the ginger group. Picrotoxin at 250 ng/rat+ ginger reduced pain sensitivity as compared to the group that received ginger, in both the early and the late phases of the formalin test. Picrotoxin at 500 ng/rat+ ginger increased pain sensitivity in the early phase and late phase of formalin test as compared to the ginger group. Conclusions: Aqueous- alcoholic extract of ginger has significant analgesic effects in late phase of formalin test and GABAA receptors may be involved in this regard.
Types of Manuscript: Original Research | Subject: Pain and addiction