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Abstract:   (290 Views)

Introduction: stroke is the major cause of long-term disability in adults. The precise role of the mTOR signaling pathway in neural viability due to Rapamycin effect in the animal model of MCAO remained elusive. Since the relationship between mTOR and miR-1, especially in neurons, is unknown, we have evaluated the effect of Rapamycin(IV) as a post-ischemic treatment on improving stroke symptoms.

Methods: Rats were divided into three groups including sham, control, and Rapamycin treatment group. Each contains four subgroups (n=7). One hour after middle cerebral artery occlusion surgery (MCAO), rats were received intravenously with 0.1 ml normal saline (NS), 0.1 ml Rapamycin in the control and treatment group, respectively. After 24 hours, neurologic deficit score (NDS), infarct volume, brain edema, and blood-brain barrier (BBB) permeability were measured in the control and treatment group. The expression of miR-1, Bcl-w, and Bad were analyzed using real-time PCR in all groups.
Results: Our results indicate that Rapamycin, significantly reduces neurological deficits, infarct volume, brain edema, and blood - brain barrier permeability. It also decreases the level of miR-1 and Bad expression and increases the level of bcl-w expression.

Conclusion: According to our findings, post-ischemic treatment with Rapamycin can be effective in improving symptoms of stroke using changing in the expression of the miR-1 gene and, consequently, a changing in the expression of the target genes of this miRNA (i.e., Bad and Bcl-w). In summary, we unravel for the first time a link between mTOR and miRNA-1 and Bcl-w and Bad in brain ischemia. 

Types of Manuscript: Original Research | Subject: Neurodegeneretive diseases